Recombinant human NMNAT3 protein (ab157274)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human NMNAT3 protein -
Biological activity
Specific Activity: 2.35 U/mg.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKSRIPVVLLACGSFNPITNMHLRMFEVARDHLHQTGMYQVIQGIISPVN DTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHH HSKLLRSPPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKD AHIQEIVEKFGLVCVGRVGHDPKGYIAESPILRMHQHNIHLAKEPVQNEI SATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQSTEGK TS -
Predicted molecular weight
30 kDa including tags -
Amino acids
1 to 252 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab157274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.79% Tris HCl, 10% Glycerol (glycerin, glycerine), 1.75% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- NaMN adenylyltransferase 3
- Nicotinamide mononucleotide adenylyltransferase 3
- Nicotinamide nucleotide adenylyltransferase 3
see all -
Function
Catalyzes the formation of NAD(+) from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency. Can use triazofurin monophosphate (TrMP) as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD(+). For the pyrophosphorolytic activity, can use NAD (+), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP(+), NADPH and NAADP(+). Protects against axonal degeneration following injury. -
Tissue specificity
Expressed in lung and spleen with lower levels in placenta and kidney. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1. -
Sequence similarities
Belongs to the eukaryotic NMN adenylyltransferase family. -
Cellular localization
Mitochondrion. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab157274 has not yet been referenced specifically in any publications.