Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human NNMT protein (Active)
See all NNMT proteins and peptides
Specific activity: 57 pmol/min/μg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 0.05% Tween 20, 1 mM DTT, 100 µM Quinoline, 100 µM SAM, 30 min, room temperature
Purity>= 90 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthFull length protein
SequenceMESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC LDGVKGDLLI DIGSGPTIYQ LLSACESFKE IVVTDYSDQN LQELEKWLKK EPEAFDWSPV VTYVCDLEGN RVKGPEKEEK LRQAVKQVLK CDVTQSQPLG AVPLPPADCV LSTLCLDAAC PDLPTYCRAL RNLGSLLKPG GFLVIMDALK SSYYMIGEQK FSSLPLGREA VEAAVKEAGY TIEWFEVISQ SYSSTMANNE GLFSLVARKL SRPL
Predicted molecular weight30 kDa
Amino acids1 to 264
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab271638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 0.99% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 22.214.171.124
- Nicotinamide N methyltransferase
- Nicotinamide N-methyltransferase
FunctionCatalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.
Tissue specificityPredominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
Sequence similaritiesBelongs to the NNMT/PNMT/TEMT family.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271638 has not yet been referenced specifically in any publications.