Recombinant Human Nodal protein (ab114307)

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SDS-PAGE - Recombinant Human Nodal protein (ab114307)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA, SDS-PAGE

    Description

    • Product name

      Recombinant Human Nodal protein

    • Expression system

      Wheat germ

    • Accession

      Q96S42

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSML YVDNGRVLLDHHKDMIVEECGC

      • Predicted molecular weight

        34 kDa including tags

      • Amino acids

        275 to 346

      • Tags

        GST tag N-Terminus

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab114307 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • HTX5
      • MGC138230
      • Nodal
      • Nodal Growth Differentiation Factor
      • Nodal homolog
      • nodal homolog (mouse)
      • Nodal, Mouse, Homolog
      • NODAL_HUMAN
      see all

    • Function

      Essential for mesoderm formation and axial patterning during embryonic development.

    • Involvement in disease

      Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.

    • Sequence similarities

      Belongs to the TGF-beta family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Nodal protein (ab114307)
      SDS-PAGE - Recombinant Human Nodal protein (ab114307)
      12.5% SDS-PAGE image showing ab114307 Stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114307? Please let us know so that we can cite the reference in this datasheet.

    ab114307 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Hello tehnical support,

    our customer is interested in Nodal protein that could be used for in vivo application in mice.

    They asked if the following AbCam products could be use for this kind of experiments?

    Nodal protein (ab114307)


    Nodal peptide (ab39951)


    Nodal peptide (ab39952)

    Thank You in advance.

    Best regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    With regards to our recombinant protein (ab114307), could you please confirm the application(s) you wish to use the product in? I can confirm that this particular recombinant protein can be used in antibody production. However, we have not tested the recombinant proteins for any activity assay yet so we do not know if the proteins are active or functional. We don’t produce the protein with endotoxin free system; therefore it is not suitable for using in cell culture system.

    We will contact you again regarding the other two products (ab39951 and ab39952) very shortly.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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