Recombinant Human Nodal protein (ab114307)

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Description

  • Product name

    Recombinant Human Nodal protein

  • Expression system

    Wheat germ

  • Accession

    Q96S42

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      RCEGECPNPVGEEFHPTNHAYIQSLLKRYQPHRVPSTCCAPVKTKPLSML YVDNGRVLLDHHKDMIVEECGC

    • Predicted molecular weight

      34 kDa including tags

    • Amino acids

      275 to 346

    • Tags

      GST tag N-Terminus

Associated products

Specifications

Our Abpromise guarantee covers the use of ab114307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

     

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • HTX5
    • MGC138230
    • Nodal
    • Nodal Growth Differentiation Factor
    • Nodal homolog
    • nodal homolog (mouse)
    • Nodal, Mouse, Homolog
    • NODAL_HUMAN
    see all

  • Function

    Essential for mesoderm formation and axial patterning during embryonic development.

  • Involvement in disease

    Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.

  • Sequence similarities

    Belongs to the TGF-beta family.

  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human Nodal protein (ab114307)
    SDS-PAGE - Recombinant Human Nodal protein (ab114307)
    12.5% SDS-PAGE image showing ab114307 Stained with Coomassie Blue.

References

ab114307 has not yet been referenced specifically in any publications.

Publishing research using ab114307? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

Hello tehnical support,

our customer is interested in Nodal protein that could be used for in vivo application in mice.

They asked if the following AbCam products could be use for this kind of experiments?

Nodal protein (ab114307)


Nodal peptide (ab39951)


Nodal peptide (ab39952)

Thank You in advance.

Best regards,

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Answer

Thank you for your enquiry and your interest in our products.

With regards to our recombinant protein (ab114307), could you please confirm the application(s) you wish to use the product in? I can confirm that this particular recombinant protein can be used in antibody production. However, we have not tested the recombinant proteins for any activity assay yet so we do not know if the proteins are active or functional. We don’t produce the protein with endotoxin free system; therefore it is not suitable for using in cell culture system.

We will contact you again regarding the other two products (ab39951 and ab39952) very shortly.

I hope this helps and if I can assist further, please do not hesitate to contact me.

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