Recombinant Human Nucleolin protein (His tag) (ab241527)
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Description
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Product name
Recombinant Human Nucleolin protein (His tag)
See all Nucleolin proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Yeast -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
VKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQK KGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPA KAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDD DSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDD EEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDE DDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGT EPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGY VDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLP YKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQG TEIDGRSISLYYTGEKGQNQDYRGGKNSTWS -
Predicted molecular weight
54 kDa including tags -
Amino acids
2 to 482 -
Tags
His tag N-Terminus -
Additional sequence information
N-terminal 6xHis-tagged
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab241527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Tris buffer, 50% Glycerol
General Info
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Alternative names
- C23
- FLJ45706
- MS1116
see all -
Function
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. -
Sequence similarities
Contains 4 RRM (RNA recognition motif) domains. -
Post-translational
modificationsSome glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. -
Cellular localization
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Nucleolin protein (His tag) (ab241527)ab241527 analyzed by (Tris-Glycine gel) discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Mass Spectrometry - Recombinant Human Nucleolin protein (His tag) (ab241527)Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241527 could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) Nucleolin.
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Mass Spectrometry - Recombinant Human Nucleolin protein (His tag) (ab241527)Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of ab241527 could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) Nucleolin.
Datasheets and documents
References
ab241527 has not yet been referenced specifically in any publications.
