Recombinant Human Nucleosome protein (Biotin) (ab216216)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus, His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Nucleosome protein (Biotin)
See all Nucleosome proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
SGRGKQGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVY LAAVLEYLTA EILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLG RVTIAQGGVLPNIQAVLLPKKT ESHHKAKGK -
Amino acids
2 to 130 -
Tags
His tag N-Terminus -
Additional sequence information
(NM_033445)
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Amino Acid Sequence 2
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Species
Human -
Sequence
PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHP DTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRL LLPGELAKHAVSEGTKAVTKYTSSK -
Amino acids
2 to 126 -
Tags
His tag N-Terminus -
Additional sequence information
(NM_003528). Contains a C-terminal Cys added as a biotinylation site.
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Amino Acid Sequence 3
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Species
Human -
Sequence
ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALRE IRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYL VGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA -
Amino acids
2 to 136 -
Tags
His tag N-Terminus -
Additional sequence information
( NM_003532)
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Amino Acid Sequence 4
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Species
Human -
Sequence
SGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLI YEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGF GG -
Amino acids
2 to 103 -
Tags
His tag N-Terminus -
Additional sequence information
(NM_003548). Total histone MW (before biotinylation) =113.8 kDa.
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Conjugation
Biotin
Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab216216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
ab216216 is Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A, H2B, H3 and H4. Each histone protein has an N-terminal His-tag. Each H2B has a C-terminal Cys added as a biotinylation site. Total histone MW (before biotinylation) = 113.8 kDa
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle. Store In the Dark.
pH: 7.50
Constituents: 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.03% EDTA, 20% Glycerol (glycerin, glycerine)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216216 has not yet been referenced specifically in any publications.