Question (29442) | Recombinant Human Occludin protein (ab114189)

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Question

Dear Sir/Madam,   We recently purchased your recombinant Occludin protein (ab114189) protein. The Cooomassie stained SDS gel provided on the product sheet shows a fairly pure form of the protein. However, when we ran the preparation on SDS page followed by western blotting using a highly specific mouse anti-occludin antibody (Invitrogen 33-1500) that always just recognized a single specific bands in cell extracts we were surprised by the result (see attached PDF). The preparation appears to be highly impure and unusable. We would like to run a Commassie gel but since the concentration of your preparation is so low we did not not want to waste so much material given high price of this product. The specific lot number is . We would like to request a proof of quality of this lot. Also, we request either a pure replacement preparation or a full refund (invoice enclosed).   We would appreciate a prompt reply in this manner.  

Answer

Thank you for contacting Abcam. I am sorry that you were having problems with ab114189, Occludin protein. I have asked the lab to redo the quality control on this product and see if there is a problem with the current whole batch of the protein, or if the problem was with the vial that was sent to you. The QC testing will take a few days to complete and so, if you would like to receive a new vial of this product then I can either send it now or we can wait for the test results. The alternative is that I can process a full refund for the original product. I look forward to your reply and helping you resolve this issue.