Question (29725) | Recombinant Human Occludin protein (ab114189)

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Question

  We recently purchased your recombinant Occludin protein (ab114189) protein. The Cooomassie stained SDS gel provided on the product sheet shows a fairly pure form of the protein. However, when we ran the preparation on SDS page followed by western blotting using a highly specific mouse anti-occludin antibody (Invitrogen 33-1500) that always just recognized a single specific bands in cell extracts we were surprised by the result (see attached PDF). The preparation appears to be highly impure and unusable. We would like to run a Commassie gel but since the concentration of your preparation is so low we did not not want to waste so much material given high price of this product. The specific lot number is 1000 420-27. We would like to request a proof of quality of this lot. Also, we request either a pure replacement preparation or a full refund (invoice enclosed).   We would appreciate a prompt reply in this manner.  

Answer

Thank you for your patience while the lab was redoing the QC testing on ab114189. I just got the result from the QC department, please see the attachment, which has a copy of the western blot.As you could see, we used the anti-OCLN antibody and anti-GST antibody in WB for the OCLN recombinant protein. Both of them could pick up a single band at 83KDa.We also re-quantified the concentration of the protein, and it is just the same as the description on the label, 0.1ug/uL. Since the original product you purchased had degraded when you went to use it, I would be happy to send you a replacement vial of the protein if you wish, or I can process a refund. I look forward to your reply and resolving this issue for you.