Recombinant human Oct4 protein (ab169842)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human Oct4 protein
    See all Oct4 proteins and peptides

  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Accession

    Q01860

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPG VGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAG VGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFA KLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKL RPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFL QCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEA AGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSV TTLGSPMHSNESGGGGSPGRRRRRRRRRRR

    • Predicted molecular weight

      41 kDa

    • Amino acids

      1 to 360

Specifications

Our Abpromise guarantee covers the use of ab169842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid

  • Additional notes

    The in vitro function was tested using specific DNA binding assays. 11R proteins were reported to successfully generate induced pluripotent stem (iPS) cells from OG2 MEFs.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.50
    Constituents: Calcium chloride, Potassium chloride, Magnesium chloride, 0.24% Tris, Glycerol, Sodium chloride

    Also contains DTT and Arginine.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Octamer binding transcription factor 4
    • MGC22487
    • Oct 3
    • Oct 4
    • Oct-3
    • Oct-4
    • OCT3
    • Oct4
    • Octamer binding protein 3
    • Octamer binding protein 4
    • Octamer binding transcription factor 3
    • Octamer-binding protein 3
    • Octamer-binding protein 4
    • Octamer-binding transcription factor 3
    • OTF 3
    • OTF 4
    • OTF-3
    • OTF3
    • OTF4
    • PO5F1_HUMAN
    • POU class 5 homeobox 1
    • POU domain class 5 transcription factor 1
    • POU domain transcription factor OCT4
    • POU domain, class 5, transcription factor 1
    • POU-type homeodomain-containing DNA-binding protein
    • POU5F1
    see all

  • Function

    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

  • Tissue specificity

    Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

  • Sequence similarities

    Belongs to the POU transcription factor family. Class-5 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • Developmental stage

    Highly expressed in undifferentiated embryonic stem cells and expression decreases gradually after embryoid body (EB) formation.

  • Domain

    The POU-specific domain mediates interaction with PKM2.

  • Post-translational
    modifications

    Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
    Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.

  • Cellular localization

    Nucleus. Expressed in a diffuse and slightly punctuate pattern.
  • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab169842? Please let us know so that we can cite the reference in this datasheet.

ab169842 has been referenced in 1 publication.

  • AlAbdi L  et al. Oct4-Mediated Inhibition of Lsd1 Activity Promotes the Active and Primed State of Pluripotency Enhancers. Cell Rep 30:1478-1490.e6 (2020). PubMed: 32023463

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