Recombinant Human Ogg1 protein (ab98249)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human Ogg1 protein
See all Ogg1 proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab98249 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHTGSMPARALLPRRMGHRTLASTPALWASIP CPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTV YRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKF QGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVT YHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQ LRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQR DYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQCR HAQEPPAKRRKGSKGPEG -
Predicted molecular weight
41 kDa including tags -
Amino acids
1 to 345 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab98249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.316% Tris HCl, 40% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
General Info
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Alternative names
- 8 hydroxyguanine DNA glycosylase
- 8 oxoguanine DNA glycosylase
- 8 oxoguanine DNA glycosylase 1
see all -
Function
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Defects in OGG1 may be a cause of renal cell carcinoma (RCC) [MIM:144700]. It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. -
Sequence similarities
Belongs to the type-1 OGG1 family. -
Cellular localization
Mitochondrion; Nucleus and Nucleus > nucleoplasm. Nucleus speckle. Nucleus matrix. Together with APEX1 is recruited to nuclear speckles in UVA-irradiated cells. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab98249 has been referenced in 2 publications.
- Schniertshauer D et al. Real-time Base Excision Repair Assay to Measure the Activity of the 8-oxoguanine DNA Glycosylase 1 in Isolated Mitochondria of Human Skin Fibroblasts. Bio Protoc 11:e3954 (2021). PubMed: 33855116
- Duan S et al. Interaction between RECQL4 and OGG1 promotes repair of oxidative base lesion 8-oxoG and is regulated by SIRT1 deacetylase. Nucleic Acids Res 48:6530-6546 (2020). PubMed: 32432680