Recombinant Human OPRT protein (His tag) (ab226883)

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SDS-PAGE - Recombinant Human OPRT protein (His tag) (ab226883)

    Key features and details

    • Expression system: Baculovirus infected insect cells
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human OPRT protein (His tag)
      See all OPRT proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      ab226883 was purified using conventional chromatography techniques.

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Baculovirus infected insect cells

    • Accession

      P11172

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLL SQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKET KDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAI VLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRF IQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKK ETNLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELIT LAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGV VKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGF ISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVG RGIISAADRLEAAEMYRKAAWEAYLSRLGVHHHHHH

      • Predicted molecular weight

        53 kDa including tags

      • Amino acids

        1 to 480

      • Tags

        His tag C-Terminus

      • Additional sequence information

        NP_000364.

    Specifications

    Our Abpromise guarantee covers the use of ab226883 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

       This product was previously labelled as UMPS

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: PBS

    General Info

    • Alternative names

      • ODC
      • OMPdecase
      • OPRT
      • OPRTase
      • Orotate phosphoribosyl transferase and orotidine 5' decarboxylase
      • Orotate phosphoribosyltransferase
      • Orotate phosphoribosyltransferase phosphoribosyltransferase
      • Orotidine 5' phosphate decarboxylase
      • Orotidine 5''-phosphate decarboxylase
      • RP11-71H17.9
      • UMP synthase
      • Umps
      • UMPS_HUMAN
      • Uridine 5' monophosphate synthase
      • Uridine monophosphate synthetase
      see all

    • Pathway

      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
      Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

    • Involvement in disease

      Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.

    • Sequence similarities

      In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
      In the C-terminal section; belongs to the OMP decarboxylase family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human OPRT protein (His tag) (ab226883)
      SDS-PAGE - Recombinant Human OPRT protein (His tag) (ab226883)

      15% SDS-PAGE analysis of 3 µg ab226883.

      MW: 50-70 kDa (SDS-PAGE under reducing conditions).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab226883? Please let us know so that we can cite the reference in this datasheet.

    ab226883 has not yet been referenced specifically in any publications.

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