Recombinant Human OPRT protein (His tag) (ab226883)

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Description

  • Product name

    Recombinant Human OPRT protein (His tag)
    See all OPRT proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    ab226883 was purified using conventional chromatography techniques.

  • Endotoxin level

    < 1.000 Eu/µg

  • Expression system

    Baculovirus infected insect cells

  • Accession

    P11172

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAVARAALGPLVTGLYDVQAFKFGDFVLKSGLSSPIYIDLRGIVSRPRLL SQVADILFQTAQNAGISFDTVCGVPYTALPLATVICSTNQIPMLIRRKET KDYGTKRLVEGTINPGETCLIIEDVVTSGSSVLETVEVLQKEGLKVTDAI VLLDREQGGKDKLQAHGIRLHSVCTLSKMLEILEQQKKVDAETVGRVKRF IQENVFVAANHNGSPLSIKEAPKELSFGARAELPRIHPVASKLLRLMQKK ETNLCLSADVSLARELLQLADALGPSICMLKTHVDILNDFTLDVMKELIT LAKCHEFLIFEDRKFADIGNTVKKQYEGGIFKIASWADLVNAHVVPGSGV VKGLQEVGLPLHRGCLLIAEMSSTGSLATGDYTRAAVRMAEEHSEFVVGF ISGSRVSMKPEFLHLTPGVQLEAGGDNLGQQYNSPQEVIGKRGSDIIIVG RGIISAADRLEAAEMYRKAAWEAYLSRLGVHHHHHH

    • Predicted molecular weight

      53 kDa including tags

    • Amino acids

      1 to 480

    • Tags

      His tag C-Terminus

    • Additional sequence information

      NP_000364.

Specifications

Our Abpromise guarantee covers the use of ab226883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid

  • Additional notes

     This product was previously labelled as UMPS

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituent: PBS

General Info

  • Alternative names

    • ODC
    • OMPdecase
    • OPRT
    • OPRTase
    • Orotate phosphoribosyl transferase and orotidine 5' decarboxylase
    • Orotate phosphoribosyltransferase
    • Orotate phosphoribosyltransferase phosphoribosyltransferase
    • Orotidine 5' phosphate decarboxylase
    • Orotidine 5''-phosphate decarboxylase
    • RP11-71H17.9
    • UMP synthase
    • Umps
    • UMPS_HUMAN
    • Uridine 5' monophosphate synthase
    • Uridine monophosphate synthetase
    see all

  • Pathway

    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.
    Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.

  • Involvement in disease

    Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1) [MIM:258900]. A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies.

  • Sequence similarities

    In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.
    In the C-terminal section; belongs to the OMP decarboxylase family.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human OPRT protein (His tag) (ab226883)
    SDS-PAGE - Recombinant Human OPRT protein (His tag) (ab226883)

    15% SDS-PAGE analysis of 3 µg ab226883.

    MW: 50-70 kDa (SDS-PAGE under reducing conditions).

References

ab226883 has not yet been referenced specifically in any publications.

Publishing research using ab226883? Please let us know so that we can cite the reference in this datasheet.

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