Description

  • Product name

    Recombinant Human Osteopontin protein
    See all Osteopontin proteins and peptides
  • Purity

    > 90 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human

Specifications

Our Abpromise guarantee covers the use of ab92964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    Mass Spectrometry

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 30% Glycerol, PBS, pH 7.4

General Info

  • Alternative names

    • BNSP
    • Bone sialoprotein 1
    • Bone sialoprotein I
    • BSP I
    • BSPI
    • Early T lymphocyte activation 1
    • ETA 1
    • ETA1
    • MGC110940
    • Nephropontin
    • OPN
    • Osteopontin
    • osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein
    • OSTP_HUMAN
    • PSEC0156
    • Secreted phosphoprotein 1
    • secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1)
    • secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1)
    • SPP 1
    • SPP-1
    • SPP1
    • SPP1/CALPHA1 fusion
    • Urinary stone protein
    • Uropontin
    see all
  • Function

    Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
    Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Tissue specificity

    Bone. Found in plasma.
  • Sequence similarities

    Belongs to the osteopontin family.
  • Post-translational
    modifications

    Extensively phosphorylated on clustered serine residues.
    N- and O-glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • ab92964 analysed by 10% SDS-PAGE stained with Coomassie Blue (CB).

References

This product has been referenced in:

  • James NE  et al. HE4 suppresses the expression of osteopontin in mononuclear cells and compromises their cytotoxicity against ovarian cancer cells. Clin Exp Immunol 193:327-340 (2018). Read more (PubMed: 29745428) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

We carry three osteopontin proteins in the catalogue.

Recombinant full length Human Osteopontin (amino acid 17-314) expressed in 293 cells, 60-65 kDa (SwissProt P10451), ab81549.

https://www.abcam.com/Osteopontin-protein-Active-ab81549.html

Recombinant full length Human Osteopontin fused to His tag at N-terminal and expressed in E. coli; amino acids 17-314 (without signal peptide) (321 amino acids), 36.2kDa. Swiss ID P10451, ab87460.

https://www.abcam.com/Osteopontin-protein-His-tag-ab87460.html

Recombinant full length Human Osteopontin Isoform B (amino acids 1-300) with MBP tag expressed in E. coli; 708 amino acids with tag, MWt 78.5 kDa (UniProt P10451-2), ab92964.

https://www.abcam.com/Osteopontin-protein-MBP-ab92964.html

I hope this information helps. Please contact me with any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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