Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human OTUB2 protein (Active)
See all OTUB2 proteins and peptides
The specific activity was determined to be ≥ 22 nmol/min/mg in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.
Purity> 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGN CFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFN AFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFF RHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMD TALNHHVFPEAATPSVYLLYKTSHYNILYAADKH
Molecular weight informationSDS-PAGE molecular weight: ~30kDa.
Amino acids1 to 234
TagsHis tag N-Terminus
Additional sequence informationGenBank: NM_023112
Our Abpromise guarantee covers the use of ab268827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.87% Sodium chloride, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine), 0.82% Sodium phosphate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Deubiquitinating enzyme OTUB2
FunctionHydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Tissue specificityWidely expressed. Expressed at higher level in brain.
Sequence similaritiesBelongs to the peptidase C65 family.
Contains 1 OTU domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268827 has not yet been referenced specifically in any publications.