Recombinant Human p21 protein (ab56278)

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Sandwich ELISA - Recombinant Human p21 protein (ab56278)
  • SDS-PAGE - Recombinant Human p21 protein (ab56278)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 80% SDS-PAGE
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE, WB

Description

  • Product name

    Recombinant Human p21 protein
    See all p21 proteins and peptides

  • Purity

    > 80 % SDS-PAGE.

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Tags

      GST tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab56278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

General Info

  • Alternative names

    • CAP20
    • CDK-interacting protein 1
    • CDKI
    • CDKN1
    • Cdkn1a
    • CDN1A_HUMAN
    • CIP1
    • Cyclin Dependent Kinase Inhibitor 1A
    • Cyclin-dependent kinase inhibitor 1
    • Cyclin-dependent kinase inhibitor 1A (P21)
    • Cyclin-dependent kinase inhibitor 1A (p21, Cip1)
    • DNA Synthesis Inhibitor
    • MDA-6
    • MDA6
    • Melanoma differentiation-associated protein
    • Melanoma differentiation-associated protein 6
    • p21
    • P21 protein
    • p21CIP1
    • p21Cip1/Waf1
    • p21WAF
    • PIC1
    • SDI1
    • SLC12A9
    • WAF1
    • Wild type p53 activated fragment 1
    • Wild type p53 activated fragment 1 (WAF1)
    • Wildtype p53-activated fragment 1
    see all

  • Function

    May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

  • Tissue specificity

    Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.

  • Sequence similarities

    Belongs to the CDI family.

  • Domain

    The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.

  • Post-translational
    modifications

    Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

Images

  • Sandwich ELISA - Recombinant Human p21 protein (ab56278)
    Sandwich ELISA - Recombinant Human p21 protein (ab56278)
    Standard curve for p21 (Analyte: ab56278); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AC8.3] to p21 (ab118) at 5µg/ml and Detector Antibody Rabbit polyclonal to p21 (ab18209) at 0.5µg/ml.
  • SDS-PAGE - Recombinant Human p21 protein (ab56278)
    SDS-PAGE - Recombinant Human p21 protein (ab56278)

    SDS-PAGE analysis of ab56278 with molecular weight markers. Approximate molecular weight 46kDa.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (2)

Publishing research using ab56278? Please let us know so that we can cite the reference in this datasheet.

ab56278 has been referenced in 2 publications.

  • Sun Z  et al. Increased SHP-1 expression results in radioresistance, inhibition of cellular senescence, and cell cycle redistribution in nasopharyngeal carcinoma cells. Radiat Oncol 10:152 (2015). PubMed: 26215037
  • Bishehsari F  et al. Anti-p21 autoantibodies detected in colorectal cancer patients: A proof of concept study. Oncoimmunology 3:e952202 (2014). PubMed: 25960931

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Question

We are interested in your recombinant p21. We have a couple questions on it: 1) what is the isoelectric point of p21 protein? 2) do you have service to modify a fluorescence label onto the protein (like 488)?

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Answer

Thank you for contacting Abcam regarding ab56278.


Regarding this p21 protein, the isoelectric point has not been determined. Currently we do not have a service for custom conjugation of proteins, however, we do sell simple 3 step kits that can be used to conjugate the protein yourself.


I would like to recommend the FITC EasyLink Conjugation kit, ab102885 along with the Purification kit ab102784 to clean up the protein buffer.


I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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Question

Hello,
Can you please tell me what the tag is and if it can be cleaved as p21 should be 21 kDa and not 47kDa.
Thank you

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Answer

Thanks again for your email and for your patience.

I've heard back from my colleagues in the lab, and the GST tag can be cleaved using a thrombin digestion protocol. We don't have a suggested digestion protocol, but there are many available online such as-

http://wolfson.huji.ac.il/purification/TagProteinPurif/CleavTombin.html

I hope this information will be useful, but please let me know if there is anything else that we can do for you and I'll be happy to help.

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Question

Can you please tell me what the tag is and if it can be cleaved as p21 should be 21 kDa and not 47kDa.
Thank you

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Answer

Thank you for contacting us with your question about ab56278.

This p21 proteincontains an N-terminal GST tag. Ihave contactedthe production labto requestmore information about a protocol to remove the GST, and I'll get back to you as soon as I have this information.

Please let me know if you have any other questions in the meantime or if there is anything else that we can do for you.

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