Overview

Description

  • Nature

    Recombinant
  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Molecular weight

      59 kDa including tags

Specifications

Our Abpromise guarantee covers the use of ab126931 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab126931 was determined to be 185 nmol/min/mg.
  • Applications

    SDS-PAGE

    Functional Studies

  • Purity

    > 90 % Densitometry.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.005% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CD5R1_HUMAN
    • CDK5 activator 1
    • CDK5P35
    • CDK5R
    • CDK5R1
    • Cyclin dependent kinase 5 regulatory subunit 1
    • Cyclin-dependent kinase 5 activator 1
    • Cyclin-dependent kinase 5 regulatory subunit 1
    • NCK 5A
    • Neuronal CDK5 activator
    • p23
    • p25
    • p35
    • Regulatory partner for CDK5 kinase
    • Tau protein kinase II 23 kDa subunit
    • TPKII regulatory subunit
    see all
  • Function

    p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII.
  • Tissue specificity

    Brain and neuron specific.
  • Sequence similarities

    Belongs to the cyclin-dependent kinase 5 activator family.
  • Post-translational
    modifications

    The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
    Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35.
  • Cellular localization

    Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites.
  • Information by UniProt

Images

  • SDS-PAGE analysis of ab126931.
  • Specific activity of ab126931 was determined to be 185 nmol/min/mg.

References

ab126931 has not yet been referenced specifically in any publications.

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