Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human p38 gamma/MAPK12 protein
See all p38 gamma/MAPK12 proteins and peptides
The specific activity of ab125651 was determined to be 220 nmol/min/mg.
Purity> 90 % Densitometry.
Purity was determined to be >90% by densitometry. Affinity purified.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight71 kDa including tags
Amino acids1 to 367
Our Abpromise guarantee covers the use of ab125651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ERK 6
FunctionResponds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Plays a role in myoblast differentiation and also in the down-regulation of cyclin D1 in response to hypoxia in adrenal cells suggesting MAPK12 may inhibit cell proliferation while promoting differentiation.
Tissue specificityHighly expressed in skeletal muscle and heart.
Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
Cellular localizationCytoplasm. Mitochondrion. Mitochondrial when associated with SH3BP5.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125651 has been referenced in 1 publication.
- Koh A et al. Microbial Imidazole Propionate Affects Responses to Metformin through p38?-Dependent Inhibitory AMPK Phosphorylation. Cell Metab 32:643-653.e4 (2020). PubMed: 32783890