Description

  • Product name

    Recombinant human p38 protein
    See all p38 proteins and peptides
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      43 kDa including tags
    • Amino acids

      1 to 360
    • Tags

      His tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab82188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form

    Liquid
  • Additional notes

    ab82188 contains no detectable proteases, DNase and RNase activity.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • CSAID binding protein
    • CSAID Binding Protein 1
    • CSAID-binding protein
    • Csaids binding protein
    • CSBP
    • CSBP 1
    • CSBP 2
    • CSBP1
    • CSBP2
    • CSPB1
    • Cytokine suppressive anti-inflammatory drug-binding protein
    • EXIP
    • MAP kinase 14
    • MAP kinase MXI2
    • MAP kinase p38 alpha
    • MAPK 14
    • MAPK14
    • MAX interacting protein 2
    • MAX-interacting protein 2
    • Mitogen Activated Protein Kinase 14
    • Mitogen activated protein kinase p38 alpha
    • Mitogen-activated protein kinase 14
    • Mitogen-activated protein kinase p38 alpha
    • MK14_HUMAN
    • Mxi 2
    • MXI2
    • p38
    • p38 ALPHA
    • p38 MAP kinase
    • p38 MAPK
    • p38 mitogen activated protein kinase
    • p38ALPHA
    • p38alpha Exip
    • PRKM14
    • PRKM15
    • RK
    • SAPK2A
    • Stress-activated protein kinase 2a
    see all
  • Function

    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificity

    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

References

ab82188 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you for contacting Abcam. I have heard from the alb about the p38 protein, ab82118. They have said it should be fine to remove the TRIS and DTT from the buffer by dialyzing against PBS. Please do the dialysis at 4C and use the protein immediately. Please let me know if there is anything else I can do to help.    

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Question
Answer

It is not known if this protein is phosphorylated or not. The recombinant protein was expressed in E. coli cells and purified using IMAC affinity column followed by SEC FPLC in Tris-HCl and KCl buffer containing 10% glycerol.   

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