Recombinant Human P4HA1 protein (ab114624)

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SDS-PAGE - Recombinant Human P4HA1 protein (ab114624)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: SDS-PAGE, WB, ELISA

    Description

    • Product name

      Recombinant Human P4HA1 protein

    • Expression system

      Wheat germ

    • Accession

      P13674

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEE DKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELEN LVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISK GNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEIS TIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEY IMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKM TPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIE IVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMR IQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRI ATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRH AACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

      • Predicted molecular weight

        85 kDa including tags

      • Amino acids

        1 to 534

    Specifications

    Our Abpromise guarantee covers the use of ab114624 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      Western blot

      ELISA

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 2-oxoglutarate-4-dioxygenase subunit alpha-1
      • 4-hydroxylase, alpha I subunit
      • 4-PH alpha-1
      • P4HA
      • P4HA1
      • P4HA1_HUMAN
      • Procollagen-proline
      • Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I
      • Prolyl 4-hydroxylase subunit alpha-1
      see all

    • Function

      Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

    • Sequence similarities

      Belongs to the P4HA family.
      Contains 1 Fe2OG dioxygenase domain.
      Contains 1 TPR repeat.

    • Cellular localization

      Endoplasmic reticulum lumen.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human P4HA1 protein (ab114624)
      SDS-PAGE - Recombinant Human P4HA1 protein (ab114624)
      ab114624 analysed on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab114624? Please let us know so that we can cite the reference in this datasheet.

    ab114624 has not yet been referenced specifically in any publications.

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