Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Product nameRecombinant Human p73 beta protein
Purity> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight88 kDa including tags
Amino acids1 to 499
Our Abpromise guarantee covers the use of ab102553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
- p53 related protein
- Tumor protein p73
RelevanceThe p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA binding domain suggest that they may have overlapping functions in the regulation of gene expression. p73 is expressed in either a full length form (a) (MW ~80kD) or a shorter (b) (MW ~70kD) mRNA variant, with exon 13 spliced out. Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53 dependent cell cycle arrest. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. p73 is upregulated in a significant fraction of anaplastic thyroid cancers, whereas it is not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab102553 has not yet been referenced specifically in any publications.