Recombinant Human p73 beta protein (ab102553)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human p73 beta protein -
Purity
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
88 kDa including tags -
Amino acids
1 to 499
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Specifications
Our Abpromise guarantee covers the use of ab102553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
General Info
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Alternative names
- p53 related protein
- P73
- TP73
- Tumor protein p73
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Relevance
The p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA binding domain suggest that they may have overlapping functions in the regulation of gene expression. p73 is expressed in either a full length form (a) (MW ~80kD) or a shorter (b) (MW ~70kD) mRNA variant, with exon 13 spliced out. Transient overexpression of p73 in cells can induce apoptosis and p21, a cellular p53 target gene primarily responsible for p53 dependent cell cycle arrest. The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections. p73 is upregulated in a significant fraction of anaplastic thyroid cancers, whereas it is not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer. -
Cellular localization
Nuclear
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab102553 has not yet been referenced specifically in any publications.