Recombinant Human PADI1 / PAD1 protein (ab125549)

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Description

  • Product name

    Recombinant Human PADI1 / PAD1 protein
    See all PADI1 / PAD1 proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Assessed by densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q9ULC6

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      95 kDa including tags

    • Amino acids

      1 to 663

Specifications

Our Abpromise guarantee covers the use of ab125549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

General Info

  • Alternative names

    • HPAD10
    • Padi1
    • PADI1_HUMAN
    • PDI
    • PDI1
    • Peptidylarginine deiminase I
    • protein arginine deiminase type I
    • Protein-arginine deiminase type I
    • Protein-arginine deiminase type-1
    see all

  • Function

    Catalyzes the deimination of arginine residues of proteins.

  • Tissue specificity

    Epidermis, prostate, testis, placenta, spleen and thymus.

  • Sequence similarities

    Belongs to the protein arginine deiminase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PADI1 / PAD1 protein (ab125549)
    SDS-PAGE - Recombinant Human PADI1 / PAD1 protein (ab125549)
    SDS-PAGE analysis of ab125549.

References

ab125549 has not yet been referenced specifically in any publications.

Publishing research using ab125549? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Question

I had two questions regarding this PAD1 product. 1. Do you assay this product for enzymatic activity? 2. Is the proprietary tag on this protein compatible with Glutathione Spin Columns for exclusion?

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Answer

The lab does not assay this product for enzymatic activity. However they have informed me that they have had customers who had done this and the activity looked good. They have found that when using this with Glutathione Spin Columns for exclusion thaey had to remove the tag, unless a thrombin digest is used.

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