Recombinant Human PADI1 / PAD1 protein (ab125549)

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SDS-PAGE - Recombinant Human PADI1 / PAD1 protein (ab125549)

    Key features and details

    • Expression system: Baculovirus infected Sf9 cells
    • Purity: > 95% SDS-PAGE
    • Suitable for: WB, SDS-PAGE

    Description

    • Product name

      Recombinant Human PADI1 / PAD1 protein
      See all PADI1 / PAD1 proteins and peptides

    • Purity

      > 95 % SDS-PAGE.
      Assessed by densitometry.

    • Expression system

      Baculovirus infected Sf9 cells

    • Accession

      Q9ULC6

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Predicted molecular weight

        95 kDa including tags

      • Amino acids

        1 to 663

    Specifications

    Our Abpromise guarantee covers the use of ab125549 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    General Info

    • Alternative names

      • HPAD10
      • Padi1
      • PADI1_HUMAN
      • PDI
      • PDI1
      • Peptidylarginine deiminase I
      • protein arginine deiminase type I
      • Protein-arginine deiminase type I
      • Protein-arginine deiminase type-1
      see all

    • Function

      Catalyzes the deimination of arginine residues of proteins.

    • Tissue specificity

      Epidermis, prostate, testis, placenta, spleen and thymus.

    • Sequence similarities

      Belongs to the protein arginine deiminase family.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PADI1 / PAD1 protein (ab125549)
      SDS-PAGE - Recombinant Human PADI1 / PAD1 protein (ab125549)
      SDS-PAGE analysis of ab125549.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab125549? Please let us know so that we can cite the reference in this datasheet.

    ab125549 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    I had two questions regarding this PAD1 product. 1. Do you assay this product for enzymatic activity? 2. Is the proprietary tag on this protein compatible with Glutathione Spin Columns for exclusion?

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    Answer

    The lab does not assay this product for enzymatic activity. However they have informed me that they have had customers who had done this and the activity looked good. They have found that when using this with Glutathione Spin Columns for exclusion thaey had to remove the tag, unless a thrombin digest is used.

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