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    recombinant-human-pag-protein-ab56521.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Recombinant Human PAG protein (ab56521)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human PAG protein (ab56521)
  • Western blot - Recombinant Human PAG protein (ab56521)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Tags: His tag N-Terminus
  • Suitable for: SDS-PAGE, WB

Description

  • Product name

    Recombinant Human PAG protein
  • Purity

    > 95 % SDS-PAGE.
    Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. > 95% (by SDS-PAGE)
  • Expression system

    Escherichia coli
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Amino acids

      41 to 432
    • Tags

      His tag N-Terminus

Associated products

  • Related Products

    • Anti-6X His tag® antibody [HIS.H8] (ab18184)
    • Anti-6X His tag® antibody [4D11] (ab5000)

Specifications

Our Abpromise guarantee covers the use of ab56521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Form

    Lyophilized
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    pH: 7.4
    Constituents: 0.164% Sodium phosphate, 0.58% Sodium chloride

  • Reconstitution
    Reconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

General Info

  • Alternative names

    • CBP
    • Csk binding protein
    • Csk-binding protein
    • FLJ37858
    • MGC138364
    • PAG
    • PAG 1
    • PAG1
    • PAG1_HUMAN
    • Phosphoprotein associated with glycosphingolipid enriched microdomains
    • Phosphoprotein associated with glycosphingolipid enriched microdomains 1
    • Phosphoprotein associated with glycosphingolipid microdomains 1
    • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
    • Protein Associated with Glycosphingolipid Enriched Microdomains
    • Transmembrane adapter protein PAG
    • Transmembrane phosphoprotein Cbp
    see all
  • Function

    Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • Tissue specificity

    Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
  • Post-translational
    modifications

    Palmitoylated.
    Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
  • Cellular localization

    Cell membrane. Present in lipid rafts.
  • Target information above from: UniProt accession Q9NWQ8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PAG protein (ab56521)
    SDS-PAGE - Recombinant Human PAG protein (ab56521)
    SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane).
  • Western blot - Recombinant Human PAG protein (ab56521)
    Western blot - Recombinant Human PAG protein (ab56521)
    Western Blot analysis of ab56521. Reducing conditions. Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab56521? Please let us know so that we can cite the reference in this datasheet.

ab56521 has been referenced in 1 publication.

  • Le Page A  et al. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell Commun Signal 12:2 (2014). WB ; Human . PubMed: 24405902

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