Recombinant Human PAG protein (ab56521)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB
Description
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Product name
Recombinant Human PAG protein -
Purity
> 95 % SDS-PAGE.
Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. > 95% (by SDS-PAGE) -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
41 to 432 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab56521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.4
Constituents: 0.164% Sodium phosphate, 0.58% Sodium chloride -
ReconstitutionReconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
General Info
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Alternative names
- CBP
- Csk binding protein
- Csk-binding protein
see all -
Function
Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. -
Tissue specificity
Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level). -
Post-translational
modificationsPalmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. -
Cellular localization
Cell membrane. Present in lipid rafts. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PAG protein (ab56521)SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane).
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Western blot - Recombinant Human PAG protein (ab56521)Western Blot analysis of ab56521. Reducing conditions. Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab56521 has been referenced in 1 publication.
- Le Page A et al. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell Commun Signal 12:2 (2014). WB ; Human . PubMed: 24405902