Recombinant Human PAG protein (ab56521)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameRecombinant Human PAG protein
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Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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SpeciesHuman
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Amino acids41 to 432
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TagsHis tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab56521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Western blot
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Purity> 95 % SDS-PAGE.
Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. > 95% (by SDS-PAGE) -
FormLyophilised
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.164% Sodium phosphate, 0.58% Sodium chloride -
ReconstitutionReconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.
General Info
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Alternative names
- CBP
- Csk binding protein
- Csk-binding protein
see all -
FunctionNegatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
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Tissue specificityUbiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
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Post-translational
modificationsPalmitoylated.
Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. -
Cellular localizationCell membrane. Present in lipid rafts.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Human PAG protein (ab56521)SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane). -
Western blot - Recombinant Human PAG protein (ab56521)Western Blot analysis of ab56521. Reducing conditions. Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.
Datasheets and documents
References
This product has been referenced in:
- Le Page A et al. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell Commun Signal 12:2 (2014). WB ; Human . Read more (PubMed: 24405902) »
