Overview

  • Product name
    Recombinant Human PAG protein
  • Protein length
    Protein fragment

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Amino acids
      41 to 432
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab56521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    Purified by Immobilized Metal Affinity Chromatography used for purification of polyhistidine-tagged recombinant proteins. > 95% (by SDS-PAGE)
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 100mM Sodium chloride, 10mM Sodium phosphate, pH 8

  • Reconstitution
    Reconstitute with 100 ul sterile deionized water. For long term storage or for preparation of more diluted working stock solution, a carrier protein (e.g. 1% serum albumin) should be added to the vial.

General Info

  • Alternative names
    • CBP
    • Csk binding protein
    • Csk-binding protein
    • FLJ37858
    • MGC138364
    • PAG
    • PAG 1
    • PAG1
    • PAG1_HUMAN
    • Phosphoprotein associated with glycosphingolipid enriched microdomains
    • Phosphoprotein associated with glycosphingolipid enriched microdomains 1
    • Phosphoprotein associated with glycosphingolipid microdomains 1
    • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1
    • Protein Associated with Glycosphingolipid Enriched Microdomains
    • Transmembrane adapter protein PAG
    • Transmembrane phosphoprotein Cbp
    see all
  • Function
    Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • Tissue specificity
    Ubiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
  • Post-translational
    modifications
    Palmitoylated.
    Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
  • Cellular localization
    Cell membrane. Present in lipid rafts.
  • Information by UniProt

Images

  • SDS-PAGE - PAG protein (His tag) (ab56521)
    SDS-PAGE - PAG protein (His tag) (ab56521)
    SDS PAGE analysis of ab56521: 12% separating gel, 6% stacking gel; reducing conditions. Staining with Coomasie Blue. Lane 1-5: PAG recombinant protein (2 µg, 1 µg, 0.5 µg, 0.2 µg, 0.1 µg per lane).
  • Western blot - PAG protein (His tag) (ab56521)
    Western blot - PAG protein (His tag) (ab56521)
    Western Blot analysis of ab56521. Reducing conditions. Lane 1-4: immunostaining with specific anti PAG antibody. Detection with Goat anti-rabbit / HRP secondary antibody. Amount of PAG recombinant protein loaded on each lane: (1) 0.1 µg, (2) 0.050 µg, (3) 0.020 µg, (4) 0.010 µg.

References

This product has been referenced in:
  • Le Page A  et al. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell Commun Signal 12:2 (2014). WB ; Human . Read more (PubMed: 24405902) »

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