Recombinant human PAI1 protein (ab92870)
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Description
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Product name
Recombinant human PAI1 protein
See all PAI1 proteins and peptides -
Biological activity
ab92870 is < 0.4% active as determined by titration with HMW tc-urokinase and SDS-PAGE. It is therefore a useful control for experiments when used in conjunction with the active from.
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
43 kDa
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab92870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 6.60
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localization
Secreted. - Information by UniProt
Images
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SDS-PAGE - Recombinant human PAI1 protein (ab92870)10% SDS-PAGE
Lane 1: PAI1 (3 ug) Reduced
Lane 2: PAI1 (3 ug) + uPA (12 ug) Reduced
Lane 3: Prestained Standard
Datasheets and documents
References
ab92870 has not yet been referenced specifically in any publications.
