Product nameRecombinant human PAK1 protein
See all PAK1 proteins and peptides
Protein lengthFull length protein
SourceBaculovirus infected Sf9 cells
Amino Acid Sequence
Molecular weight95 kDa including tags
Amino acids1 to 545
Our Abpromise guarantee covers the use of ab125805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Biological activityThe specific activity of ab125805 was determined to be 68 - 74 nmol/min/mg.
Purity> 95 % Densitometry.
Purity was determined to be >95% by densitometry.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Alpha PAK
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
- Information by UniProt
ab125805 has not yet been referenced specifically in any publications.