Recombinant Human PAPSS1 protein (ab133001)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human PAPSS1 protein -
Expression system
Wheat germ -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMA LEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFA DAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVK GLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQ ERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLA EGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLD GCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVME QGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLR NPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQ HAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVG RDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKR MDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSL EKA -
Predicted molecular weight
95 kDa including tags -
Amino acids
22 to 624
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Specifications
Our Abpromise guarantee covers the use of ab133001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1
- 3' phosphoadenosine 5' phosphosulfate synthase 1
- 3''-phosphoadenosine-5''-phosphosulfate synthase
see all -
Function
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. -
Tissue specificity
Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage. -
Pathway
Sulfur metabolism; sulfate assimilation. -
Sequence similarities
In the N-terminal section; belongs to the APS kinase family.
In the C-terminal section; belongs to the sulfate adenylyltransferase family. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human PAPSS1 protein (ab133001)12.5% SDS-PAGE analysis of ab133001 stained with Coomassie Blue.
Datasheets and documents
References
ab133001 has not yet been referenced specifically in any publications.
