Recombinant Human PAPSS1 protein (ab133001)

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SDS-PAGE - Recombinant Human PAPSS1 protein (ab133001)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human PAPSS1 protein

    • Expression system

      Wheat germ

    • Accession

      O43252

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMA LEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFA DAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVK GLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQ ERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLA EGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLD GCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVME QGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLR NPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQ HAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVG RDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKR MDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSL EKA

      • Predicted molecular weight

        95 kDa including tags

      • Amino acids

        22 to 624

    Specifications

    Our Abpromise guarantee covers the use of ab133001 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid

    • Additional notes

       

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1
      • 3' phosphoadenosine 5' phosphosulfate synthase 1
      • 3''-phosphoadenosine-5''-phosphosulfate synthase
      • Adenosine-5''-phosphosulfate 3''-phosphotransferase
      • Adenylyl-sulfate kinase
      • Adenylylsulfate 3''-phosphotransferase
      • APS kinase
      • ATP-sulfurylase
      • ATPSK 1
      • ATPSK1
      • ATPSK2
      • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthetase 1
      • PAPS synthase 1
      • PAPS synthetase 1
      • PAPS1_HUMAN
      • PAPSS
      • PAPSS 1
      • PAPSS1
      • SAT
      • SK 1
      • SK1
      • SK2
      • Sulfate adenylate transferase
      • Sulfurylase kinase 1
      see all

    • Function

      Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

    • Tissue specificity

      Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.

    • Pathway

      Sulfur metabolism; sulfate assimilation.

    • Sequence similarities

      In the N-terminal section; belongs to the APS kinase family.
      In the C-terminal section; belongs to the sulfate adenylyltransferase family.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PAPSS1 protein (ab133001)
      SDS-PAGE - Recombinant Human PAPSS1 protein (ab133001)
      12.5% SDS-PAGE analysis of ab133001 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab133001? Please let us know so that we can cite the reference in this datasheet.

    ab133001 has not yet been referenced specifically in any publications.

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