Recombinant Human PAPSS1 protein (ab133001)

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Overview

  • Product name

    Recombinant Human PAPSS1 protein

  • Protein length

    Protein fragment

Description

  • Nature

    Recombinant

  • Source

    Wheat germ
  • Amino Acid Sequence
    • Accession
      O43252

    • Species

      Human

    • Sequence

      MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMA LEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFA DAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVK GLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQ ERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLA EGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLD GCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVME QGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLR NPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQ HAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVG RDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKR MDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSL EKA

    • Molecular weight

      95 kDa including tags

    • Amino acids

      22 to 624

Specifications

Our Abpromise guarantee covers the use of ab133001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    Protein concentration is above or equal to 0.05 µg/µl.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • 3 prime phosphoadenosine 5 prime phosphosulfate synthase 1
    • 3' phosphoadenosine 5' phosphosulfate synthase 1
    • 3''-phosphoadenosine-5''-phosphosulfate synthase
    • Adenosine-5''-phosphosulfate 3''-phosphotransferase
    • Adenylyl-sulfate kinase
    • Adenylylsulfate 3''-phosphotransferase
    • APS kinase
    • ATP-sulfurylase
    • ATPSK 1
    • ATPSK1
    • ATPSK2
    • Bifunctional 3' phosphoadenosine 5' phosphosulfate synthetase 1
    • PAPS synthase 1
    • PAPS synthetase 1
    • PAPS1_HUMAN
    • PAPSS
    • PAPSS 1
    • PAPSS1
    • SAT
    • SK 1
    • SK1
    • SK2
    • Sulfate adenylate transferase
    • Sulfurylase kinase 1
    see all

  • Function

    Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. Also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.

  • Tissue specificity

    Expressed in testis, pancreas, kidney, thymus, prostate, ovary, small intestine, colon, leukocytes and liver. Also expressed in high endothelial venules (HEV) cells and in cartilage.

  • Pathway

    Sulfur metabolism; sulfate assimilation.

  • Sequence similarities

    In the N-terminal section; belongs to the APS kinase family.
    In the C-terminal section; belongs to the sulfate adenylyltransferase family.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PAPSS1 protein (ab133001)
    SDS-PAGE - Recombinant Human PAPSS1 protein (ab133001)
    12.5% SDS-PAGE analysis of ab133001 stained with Coomassie Blue.

References

ab133001 has not yet been referenced specifically in any publications.

Publishing research using ab133001? Please let us know so that we can cite the reference in this datasheet.

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