Recombinant Human PAR6 protein (ab132012)

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SDS-PAGE - Recombinant Human PAR6 protein (ab132012)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human PAR6 protein

    • Expression system

      Wheat germ

    • Accession

      Q9NPB6-2

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRASVSGFQEFSRLLRAVH QIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPPPLRLLVQKREADSS GLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLL PETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGG LAESTGLLAVSDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQ RNNVVRGASGRLTGPPSAGPGPAEPDSDDDSSDLVIENRQPPSSNGLSQG PPCWDLHPGCRHPGTRSSLPSLDDQEQASSGWGSRIRGDGSGFSL

      • Predicted molecular weight

        64 kDa including tags

      • Amino acids

        1 to 345

    Specifications

    Our Abpromise guarantee covers the use of ab132012 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 0710008C04Rik
      • 2610010A15Rik
      • Par 6 partitioning defective 6 C elegans homolog alpha
      • Par 6 partitioning defective 6 homolog alpha
      • Par 6 partitioning defective 6 homolog alpha C elegans
      • PAR 6A
      • PAR-6
      • PAR-6 alpha
      • par-6 family cell polarity regulator alpha
      • PAR-6A
      • PAR6
      • PAR6A_HUMAN
      • PAR6alpha
      • PAR6C
      • Pard6a
      • Partitioning defective 6 homolog alpha
      • partitioning-defective protein 6
      • partitioning-defective protein 6, C. elegans, homolog of, alpha
      • Tax interacting protein 40
      • Tax interaction protein 40
      • TAX40
      • TIP 40
      • TIP-40
      • TIP40
      see all

    • Function

      Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.

    • Tissue specificity

      Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.

    • Sequence similarities

      Belongs to the PAR6 family.
      Contains 1 OPR domain.
      Contains 1 PDZ (DHR) domain.
      Contains 1 pseudo-CRIB domain.

    • Domain

      The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
      The OPR domain mediates interactions with MAP2K5.
      The PDZ domain mediates the interaction with CRB3.

    • Cellular localization

      Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human PAR6 protein (ab132012)
      SDS-PAGE - Recombinant Human PAR6 protein (ab132012)
      12.5% SDS-PAGE stained with Coomassie Blue showing ab132012 at approximately 63.7 kDa.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab132012? Please let us know so that we can cite the reference in this datasheet.

    ab132012 has not yet been referenced specifically in any publications.

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