Overview

  • Product name

    Recombinant Human PARN protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MEIIRSNFKSNLHKVYQAIEEADFFAIDGEFSGISDGPSVSALTNGFDTP EERYQKLKKHSMDFLLFQFGLCTFKYDYTDSKYITKSFNFYVFPKPFNRS SPDVKFVCQSSSIDFLASQGFDFNKVFRNGIPYLNQEEERQLREQYDEKR SQANGAGALSYVSPNTSKCPVTIPEDQKKFIDQVVEKIEDLLQSEENKNL DLEPCTGFQRKLIYQTLSWKYPKGIHVETLETEKKERYIVISKVDEEERK RREQQKHAKEQEELNDAVGFSRVIHAIANSGKLVIGHNMLLDVMHTVHQF YCPLPADLSEFKEMTTCVFPRLLDTKLMASTQPFKDIINNTSLAELEKRL KETPFNPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSF LSPPKIHVSARSKLIEPFFNKLFLMRVMDIPYLNLEGPDLQPKRDHVLHV TFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVKIAVNTSKY AESYRIQTYAEYMGRKQEEKQIKRKWTEDSWKEADSKRLNPQCIPYTLQN HYYRNNSFTAPSTVGKRNLSPSQEEAGLEDGVSGEISDTELEQTDSCAEP LSEGRKKAKKLKRMKKELSPAGSISKNSPATLFEVPDTW
    • Molecular weight

      96 kDa including tags
    • Amino acids

      1 to 639

Specifications

Our Abpromise guarantee covers the use of ab114690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    ELISA

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • DAN
    • Deadenylating nuclease
    • Deadenylation nuclease
    • PARN
    • PARN_HUMAN
    • Poly A specific ribonuclease
    • Poly(A) specific ribonuclease
    • Poly(A)-specific ribonuclease PARN
    • Polyadenylate specific ribonuclease
    • Polyadenylate-specific ribonuclease
    see all
  • Function

    3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the CAF1 family.
    Contains 1 R3H domain.
  • Cellular localization

    Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
  • Information by UniProt

Images

  • ab114690 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

References

ab114690 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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