Key features and details
- Expression system: Baculovirus infected Sf21 cells
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameRecombinant Human PARP1 (mutated E988K) protein
See all PARP1 proteins and peptides
Purity> 95 % SDS-PAGE.
Expression systemBaculovirus infected Sf21 cells
Protein lengthFull length protein
Amino acids1 to 1014
Additional sequence informationHuman full-length inactive mutant E988K of PARP1 fused to a His-tag.
Our Abpromise guarantee covers the use of ab157027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity: 0.5% of wild type PARP1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.7% Imidazole
Constituents: 0.16% Tris HCl, 10% Glycerol, 1.75% Sodium chloride, 0.02% Beta mercaptoethanol, 0.2% Nonylphenol, ethoxylated
- ADP ribosyltransferase
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
- ADP ribosyltransferase diphtheria toxin like 1
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab157027 has not yet been referenced specifically in any publications.