Key features and details
- Expression system: Baculovirus
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PARP1 protein
See all PARP1 proteins and peptides
Biological activity1000 U/vial, specific activity = 20000 U/mg PARP1. 1U=10 fmol ADP-ribose incorporated into 5 µg immobilized histone in 30 min at room temperature. Note: Activity measurements are approximate values.
Purity> 95 % SDS-PAGE.
Protein lengthFull length protein
Predicted molecular weight116 kDa
Amino acids1 to 1014
TagsDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab123834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
STORAGE CONDITIONS: Store reconstituted solution at -70°C. Avoid multiple freeze-thaw cycles. CAUTION: There is loss of PARP1 enzymatic activity upon each free/thaw cycle. It is suggested to aliquot the reconstituted enzyme into multiple tubes and freeze at -70°C. Alternatively, add glycerol at 1:1 vol/vol to the reconstituted PARP1, mix gently by trituration, and store at -20°C (do not store in a frost-free freezer!) for up to 6 months.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C.
Constituents: 0.02% DTT, 0.24% Tris, 0.003% EDTA, 1.74% Sodium chloride
Contains lyophilization stabilizers.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionSpin tube in a microfuge for 15 sec to sediment lyophilized material. Carefully open the vial and add 100 µL dH2O. Vortex gently for 20 sec (avoid air bubbles). Let stand for 5 min. Carefully triturate the sample 10-times using a pipetman (avoid air bubbles). Spin briefly in microfuge to consolidate.
- ADP ribosyltransferase
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
- ADP ribosyltransferase diphtheria toxin like 1
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123834 has not yet been referenced specifically in any publications.