Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
      P09874
    • Species
      Human
    • Sequence
      SADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKK EVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAE VKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMK
    • Molecular weight
      15 kDa
    • Amino acids
      385 to 524
    • Additional sequence information
      Fused to a His-tag.

Specifications

Our Abpromise guarantee covers the use of ab157069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Purity
    >= 99 % SDS-PAGE.
    ab157069 was affinity purified on Ni-beads.
  • Form
    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Preservative: 0.41% Imidazole
    Constituents: 0.009% PMSF, 0.79% Tris HCl, 10% Glycerol, 1.17% Sodium chloride

General Info

  • Alternative names
    • ADP ribosyltransferase
    • ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
    • ADP ribosyltransferase diphtheria toxin like 1
    • ADP ribosyltransferase NAD(+)
    • ADPRT
    • ADPRT 1
    • ADPRT1
    • ARTD1
    • msPARP
    • NAD(+) ADP ribosyltransferase 1
    • NAD(+) ADP-ribosyltransferase 1
    • pADPRT 1
    • pADPRT1
    • PARP
    • PARP 1
    • PARP-1
    • PARP1
    • PARP1_HUMAN
    • Poly (ADP ribose) polymerase 1
    • poly (ADP ribose) polymerase family, member 1
    • Poly [ADP-ribose] polymerase 1
    • Poly(ADP ribose) polymerase
    • poly(ADP ribose) synthetase
    • poly(ADP ribosyl)transferase
    • Poly[ADP ribose] synthetase 1
    • Poly[ADP-ribose] synthase 1
    • PPOL
    see all
  • Function
    Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 PARP alpha-helical domain.
    Contains 1 PARP catalytic domain.
    Contains 2 PARP-type zinc fingers.
  • Post-translational
    modifications
    Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
    S-nitrosylated, leading to inhibit transcription regulation activity.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PARP1 protein (ab157069)
    SDS-PAGE - Recombinant Human PARP1 protein (ab157069)

    Total cell extract from E. coli BL21 cells before (lane 1) and after (lane 2) induction of the expression of Human PARP1 ab157069 with 0.5mM IPTG for 2 hours. Lanes 3-5: Purified Human PARP1 (BRCT Domain).

References

ab157069 has not yet been referenced specifically in any publications.

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