Recombinant Human PARP1 protein (ab157069)
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Overview
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Product nameRecombinant Human PARP1 protein
See all PARP1 proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceSADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKK EVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAE VKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMK
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Molecular weight15 kDa
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Amino acids385 to 524
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Additional sequence informationFused to a His-tag.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab157069 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Purity>= 99 % SDS-PAGE.
ab157069 was affinity purified on Ni-beads. -
FormLiquid
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Preservative: 0.41% Imidazole
Constituents: 0.009% PMSF, 0.79% Tris HCl, 10% Glycerol, 1.17% Sodium chloride
General Info
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Alternative names
- ADP ribosyltransferase
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
- ADP ribosyltransferase diphtheria toxin like 1
see all -
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
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Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers. -
Post-translational
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity. -
Cellular localizationNucleus.
- Information by UniProt
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References
ab157069 has not yet been referenced specifically in any publications.