Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus, Avi tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PARP1 protein (Active)
See all PARP1 proteins and peptides
Enzyme reaction is conducted using a PARP Assay Kit at room temperature for 1 hour.
Purity>= 90 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight117 kDa
Amino acids1 to 1014
TagsDDDDK tag N-Terminus , Avi tag N-Terminus
Our Abpromise guarantee covers the use of ab271649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine), 0.01% TCEP
80 µg/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ADP ribosyltransferase
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase)
- ADP ribosyltransferase diphtheria toxin like 1
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similaritiesContains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
modificationsPhosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271649 has not yet been referenced specifically in any publications.