Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PARP14 protein
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form. Enzyme reaction is conducted using a PARP14 Assay Kit at room temperature for 1 hour.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceMHHHHHHGGGSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKW RNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAE ISMLEGAVLDIRYGVSRIAYSKDFETLVDFLSKLPEMLKMFKDRLCHKTY LNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKY LKSSKYIAWPLQGWQATFGGGDHPPKSDPENLYFQGGGEFKEYQELNELQ KKLNNISLDHKRPLIKVLGISRDVMQARDEIEAMIKRVRLAKEQESRADC ISEFIEWQYNDNNTSHCFNKMTNLKLEDARREKKKTVDVKINHRHYTVNL NTYTATDTKGHSLSVQRLTKSKVDIPAHWSDKQQNFCVVELLPSDPEYNT VASKFNQTCSHFRIEKIERIQNPDLWNSYQAKKKTMDAKNGQTMNEKQLF HGTDAGSVPHVNRNGFNRSYAGKNAVAYGKGTYFAVNANYSANDTYSRPD ANGRKHVYVRVLTGIYTHGNHSLIVPPSKNPQNPTDLYDTVTDNVHHPSL FVAFYDYQAYPEYLITFRK
Predicted molecular weight67 kDa including tags
Amino acids1470 to 1801
TagsHis tag N-Terminus , GST tag N-Terminus
Our Abpromise guarantee covers the use of ab198628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Preservative: 1.28% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol, 0.05% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ADP-ribosyltransferase diphtheria toxin-like 8
- B aggressive lymphoma protein 2
FunctionEnhances STAT6-dependent transcription (By similarity). Has ADP-ribosyltransferase activity.
Sequence similaritiesContains 3 Macro domains.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain.
Cellular localizationNucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198628 has not yet been referenced specifically in any publications.