Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 12% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PARP2 protein
See all PARP2 proteins and peptides
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Assay Conditions: Enzyme reaction is conducted using a PARP2 Assay Kit at room temperature for 1 hour.
Purity>= 12 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceAARRRRSTGGGRARALNESKRVNNGNTAPEDSSPAKKTRRCQRQESKKMP VAGGKANKDRTEDKQDGMPGRSWASKRVSESVKALLLKGKAPVDPECTAK VGKAHVYCEGNDVYDVMLNQTNLQFNNNKYYLIQLLEDDAQRNFSVWMRW GRVGKMGQHSLVACSGNLNKAKEIFQKKFLDKTKNNWEDREKFEKVPGKY DMLQMDYATNTQDEEETKKEESLKSPLKPESQLDLRVQELIKLICNVQAM EEMMMEMKYNTKKAPLGKLTVAQIKAGYQSLKKIEDCIRAGQHGRALMEA CNEFYTRIPHDFGLRTPPLIRTQKELSEKIQLLEALGDIEIAIKLVKTEL QSPEHPLDQHYRNLHCALRPLDHESYEFKVISQYLQSTHAPTHSDYTMTL LDLFEVEKDGEKEAFREDLHNRMLLWHGSRMSNWVGILSHGLRIAPPEAP ITGYMFGKGIYFADMSSKSANYCFASRLKNTGLLLLSEVALGQCNELLEA NPKAEGLLQGKHSTKGLGKMAPSSAHFVTLNGSTVPLGPASDTGILNPDG YTLNYNEYIVYNPNQVRMRYLLKVQFNFLQLW
Predicted molecular weight92 kDa including tags
Amino acids2 to 583
TagsGST tag N-Terminus
Additional sequence informationNM_005484.
Our Abpromise guarantee covers the use of ab198766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.49% Glutathione, 20% Glycerol, 0.05% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ADP ribosyltransferase like 2
- ADP-ribosyltransferase diphtheria toxin-like 2
- ADPRT 2
FunctionInvolved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.
Tissue specificityWidely expressed, mainly in actively dividing tissues. The highest levels are in the brain, heart, pancreas, skeletal muscle and testis; also detected in kidney, liver, lung, placenta, ovary and spleen; levels are low in leukocytes, colon, small intestine, prostate and thymus.
Sequence similaritiesContains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
modificationsPoly-ADP-ribosylated by PARP1.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198766 has been referenced in 1 publication.
- Müller KH et al. Poly(ADP-Ribose) Links the DNA Damage Response and Biomineralization. Cell Rep 27:3124-3138.e13 (2019). PubMed: 31189100