Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 33% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human PARP7 protein (Tagged)
Specific Activity: >40 pmol/min/μg
Assay Conditions: Assay was done according to PARP7 assay kit protocol. First, histone proteins are coated on a 96-well plate. Next, the biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Purity>= 33 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceKRRPLFRSCF ILLPYLQTLG GVPTQAPPPL EATSSSQIIC PDGVTSANFY PETWVYMHPS QDFIQVPVSA EDKSYRIIYN LFHKTVPEFK YRILQILRVQ NQFLWEKYKR KKEYMNRKMF GRDRIINERH LFHGTSQDVV DGICKHNFDP RVCGKHATMF GQGSYFAKKA SYSHNFSKKS SKGVHFMFLA KVLTGRYTMG SHGMRRPPPV NPGSVTSDLY DSCVDNFFEP QIFVIFNDDQ SYPYFVIQYE EVSNTVSI
Predicted molecular weight31 kDa including tags
Amino acids400 to 657
TagsDDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab271651 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
100 µg/mL DDDDK peptide.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- ADP-ribosyltransferase diphtheria toxin-like 14
FunctionPoly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
Sequence similaritiesContains 1 C3H1-type zinc finger.
Contains 1 PARP catalytic domain.
Contains 1 WWE domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271651 has not yet been referenced specifically in any publications.