Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE, Functional Studies, GSA
Product nameRecombinant Human PC4 (mutated S13 + S15 + S17 + S19) protein
See all PC4 proteins and peptides
Biological activity1 unit equals 1 nanogram of purified protein.
Purity> 95 % SDS-PAGE.
ab81957 is purified by conventional chromatography.
Expression systemEscherichia coli
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab81957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Gel supershift assays
Wild-type PC4, but not the kinase inhibitory-deficient mutant of PC4 (serine mutations), represses specific transcription in vivo.1 unit equals 1 nanogram of purified protein.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)
- Activated RNA polymerase II transcriptional coactivator p15
- Interferon related developmental regulator 1
FunctionGeneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
Sequence similaritiesBelongs to the transcriptional coactivator PC4 family.
modificationsActivity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab81957 has not yet been referenced specifically in any publications.