Description

  • Product name

    Recombinant Human PC4 (mutated S13 + S15 + S17 + S19) protein
    See all PC4 proteins and peptides
  • Biological activity

    1 unit equals 1 nanogram of purified protein.
  • Purity

    > 95 % SDS-PAGE.
    ab81957 is purified by conventional chromatography.
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Modifications

      mutated S

Specifications

Our Abpromise guarantee covers the use of ab81957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    EMSA

  • Form

    Liquid
  • Additional notes


    Wild-type PC4, but not the kinase inhibitory-deficient mutant of PC4 (serine mutations), represses specific transcription in vivo.

    1 unit equals 1 nanogram of purified protein.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol

General Info

  • Alternative names

    • Activated RNA polymerase II transcriptional coactivator p15
    • Interferon related developmental regulator 1
    • MGC102747
    • p14
    • P15
    • PC4
    • PC4 LSB
    • Positive cofactor 4
    • RPO2TC1
    • Sub1
    • SUB1 homolog
    • TCP4_HUMAN
    see all
  • Function

    General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • Sequence similarities

    Belongs to the transcriptional coactivator PC4 family.
  • Post-translational
    modifications

    Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • Cellular localization

    Nucleus.
  • Information by UniProt

References

ab81957 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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