Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Suitable for: SDS-PAGE, WB, ELISA, Dot blot, Functional Studies
Product nameRecombinant Human PCNA protein
See all PCNA proteins and peptides
Purity> 98 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceMFEARLVQGSILKKVLEALKDLINEACWDISSSGVNLQSMDSSHVSLVQL TLRSEGFDTYRCDRNLAMGVNLTSMSKILKCAGNEDIITLRAEDNADTLA LVFEAPNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMPSGEFARICRD LSHIGDAVVISCAKDGVKFSASGELGNGNIKLSQTSNVDKEEEAVTIEMN EPVQLTFALRYLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMGHLKYY LAPKIEDEEGS
Predicted molecular weight29 kDa
Amino acids1 to 261
Our Abpromise guarantee covers the use of ab172197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -80°C. Stable for 12 months at -20°C.
Constituents: 0.6% HEPES, 0.03% EDTA, 0.01% 4-Nonylphenol, branched, ethoxylated, 0.02% DTT, 0.0002% Leupeptin, 0.002% PMSF, 0.44% Sodium chloride, 50% Glycerol (glycerin, glycerine)
FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Sequence similaritiesBelongs to the PCNA family.
modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
- Information by UniProt
All lanes : anti-PCNA
Lane 1 :
Recombinant Human PCNA protein (ab172197) at 0.00300000002607703 µg
Lane 2 :
Recombinant Human PCNA protein (ab172197) at 0.00100000004749745 µg
Lane 3 :
Recombinant Human PCNA protein (ab172197) at 0.000300000014249235 µg
Lane 4 : HeLa cell lysate at 10 µg
Lane 5 : HeLa cell lysate at 2 µg
Predicted band size: 29 kDa
ab172197 is greater than 98% pure as determined by SDS-PAGE (CBB staining).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab172197 has been referenced in 1 publication.
- Tsurimoto T et al. Human Werner helicase interacting protein 1 (WRNIP1) functions as a novel modulator for DNA polymerase delta. Genes Cells 10:13-22 (2005). PubMed: 15670210