Recombinant Human PD-L1 protein (ab216206)

Submit a review Submit a question

Description

  • Product name

    Recombinant Human PD-L1 protein
    See all PD-L1 proteins and peptides

  • Purity

    > 90 % SDS-PAGE.
    Purity measured by gel filtration.

  • Expression system

    HEK 293 cells

  • Accession

    Q9NZQ7

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFV HGEEDLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRCMISY GGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAEGYPKAEVIWT SSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEE NHTAELVIPELPLAHPPNERTDYKDDDDK

    • Predicted molecular weight

      26 kDa including tags

    • Amino acids

      19 to 239

    • Tags

      DDDDK tag C-Terminus

Specifications

Our Abpromise guarantee covers the use of ab216206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.4
    Constituents: 78% Phosphate Buffer, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol

General Info

  • Alternative names

    • B7 H
    • B7 H1
    • B7 homolog 1
    • B7-H1
    • B7H
    • B7H1
    • CD 274
    • CD274
    • CD274 antigen
    • CD274 molecule
    • MGC142294
    • MGC142296
    • OTTHUMP00000021029
    • PD L1
    • PD-L1
    • PD1L1_HUMAN
    • PDCD1 ligand 1
    • PDCD1L1
    • PDCD1LG1
    • PDL 1
    • PDL1
    • Programmed cell death 1 ligand 1
    • Programmed death ligand 1
    • RGD1566211
    see all

  • Function

    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

  • Tissue specificity

    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane and Endomembrane system.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PD-L1 protein (ab216206)
    SDS-PAGE - Recombinant Human PD-L1 protein (ab216206)

    4-20% SDS-PAGE analysis of ab216206 (3 μg) with Coomassie staining.

  • Functional Studies - Recombinant Human PD-L1 protein (ab216206)
    Functional Studies - Recombinant Human PD-L1 protein (ab216206)

    Assay Conditions: 10 nM PD-1, Biotinlabeled was mixed with varying amounts of PD-L1, FLAG-tag ab216206 in PD-1 Assay Buffer for 1 hr at RT. FLAG-acceptor beads were added and incubated for 30 min at RT followed by strep-conjugated donor beads for 30 min at RT. Alpha counts were read.

  • Other - Recombinant Human PD-L1 protein (ab216206)
    Other - Recombinant Human PD-L1 protein (ab216206)

    Gel Filtration Curve.

References

ab216206 has not yet been referenced specifically in any publications.

Publishing research using ab216206? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab216206.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com