Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human PD-L1 protein (Tagged)
See all PD-L1 proteins and peptides
Assay Conditions: Add 50 uL of PD-1-Biotin in assay buffer (PBS with 0.1% BSA) to the wells precoated with PD-L1 on a white maxisorp plate. Incubate the reaction mixture at room temperature for 2 hours. Wash and block wells. Then add streptavidin-HRP and incubate for 1 hour. Finally, add HRP chemiluminescent substrates and read luminescence.
Purity>= 90 % SDS-PAGE.
Expression systemHEK 293 cells
Protein lengthProtein fragment
SequenceFTVTVPKDLY VVEYGSNMTI ECKFPVEKQL DLAALIVYWE MEDKNIIQFV HGEEDLKVQH SSYRQRARLL KDQLSLGNAA LQITDVKLQD AGVYRCMISY GGADYKRITV KVNAPYNKIN QRILVVDPVT SEHELTCQAE GYPKAEVIWT SSDHQVLSGK TTTTNSKREE KLFNVTSTLR INTTTNEIFY CTFRRLDPEE NHTAELVIPE LPLAHPPNER T
Predicted molecular weight53 kDa including tags
Amino acids19 to 239
TagsFc tag C-Terminus
Additional sequence informationMouse IgG2a Fc
Our Abpromise guarantee covers the use of ab271693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- B7 H
- B7 H1
- B7 homolog 1
FunctionInvolved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Tissue specificityHighly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Sequence similaritiesBelongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell membrane and Endomembrane system.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271693 has not yet been referenced specifically in any publications.