Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      LDSPDR PWNPPTFSPA LLVVTEGDNA TFTCSFSNTS ESFVLNWYRM SPSNQTDKLA AFPEDRSQPG QDCRFRVTQL PNGRDFHMSV VRARRNDSGT YLCGAISLAP KAQIKESLRA ELRVTERRAE VPTAHPSPSP RPAGQFQ
    • Molecular weight

      17 kDa including tags
    • Amino acids

      25 to 167
    • Tags

      His tag C-Terminus
    • Additional sequence information

      (NP_005009.2)

Specifications

Our Abpromise guarantee covers the use of ab174035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Measured by its binding ability in a functional ELISA.

    Immobilized PD1 at 10 μg/ml (100 μl/well) can bind recombinant Human B7-H1 Fc chimera with a linear range of 0.005- 0.4 μg/ml.

  • Applications

    HPLC

    SDS-PAGE

    Functional Studies

    ELISA

  • Endotoxin level

    < 1.000 Eu/µg
  • Purity

    > 95 % SDS-PAGE.
    >90% as determined by SEC-HPLC.
  • Form

    Lyophilised
  • Additional notes

    For long term storage, store at lyophlized state at -20°C or lower. After reconstitution, store under sterile conditions for 3 months at -70°C or 12 months at 4-8°C at lyophlized state. Avoid repeated freeze-thaw cycles.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -20°C or -80°C. Please see notes section.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in 100 µl sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD279
    • CD279 antigen
    • hPD 1
    • hPD l
    • hPD-1
    • hSLE1
    • PD 1
    • PD-1
    • PD1
    • PDCD 1
    • PDCD1
    • PDCD1_HUMAN
    • Programmed cell death 1
    • Programmed cell death 1 protein
    • Programmed cell death protein 1
    • Protein PD 1
    • Protein PD-1
    • SLEB2
    • Systemic lupus erythematosus susceptibility 2
    see all
  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Immobilized ab174035 at 0.2μg/mL (100 μL/well) binds Human PD-L1, Fc Tag.

    Linear range of 0.31-1.25 μg/mL (QC tested).

  • Immobilized ab174035 at 0.2 μg/mL ( 100 μl/well ) binds Human PD-L2, Fc Tag. 

    Linear range of 0.16-2.5 μg/mL (QC tested).

  • Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Human PD-L2, Mouse IgG1 Fc Tag.

    Linear range of 10-156 ng/mL (Routinely tested).

  • Immobilized ab174035 at 2 μg/mL (100 μL/well) binds Nivolumab.

    Linear range of 0.1-3 ng/mL (Routinely tested).

  • Opdivo(Nivolumab), captured on CM5 chip via anti-human IgG Fc antibodies surface, binds ab174035 with an affinity constant of 4.94 nM, as determined in an SPR assay (Biacore T200) (Routinely tested).

  • Reduced ab174035 on SDS-PAGE, stained overnight with Coomassie Blue.

    The purity of the protein is greater than 95%.

    The protein migrates as 31-44kDa under reducing conditions, due to glycosylation.

  • The purity of ab174035 was greater than 90%, as determined by SEC-HPLC.

References

ab174035 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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