Recombinant human PD1 protein (Fc Chimera Active) (ab221398)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.100 Eu/g
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, HPLC
Description
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Product name
Recombinant human PD1 protein (Fc Chimera Active)
See all PD1 proteins and peptides -
Biological activity
Immobilized Human PD-L1, His Tag at 1 μg/mL ( 100 μL/well ) can bind ab221398 with a linear range of 0.1-3 ng/mL.
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Purity
> 95 % SDS-PAGE.
>90% as determined by SEC-HPLC. Protein A purified. -
Endotoxin level
< 0.100 Eu/g -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSN QTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCG AISLAPKAQIKESLRAELRVTERRAEVPTAHPSPSPRPAGQFQ -
Predicted molecular weight
43 kDa including tags -
Amino acids
25 to 167 -
Tags
Fc tag C-Terminus -
Additional sequence information
This protein carries a human IgG1 Fc tag at the C-terminus.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab221398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
HPLC
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Form
Lyophilized -
Additional notes
Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute with sterile deionized water to a concentration of 400 µg/ml.
General Info
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Alternative names
- CD279
- CD279 antigen
- hPD 1
see all -
Function
Possible cell death inducer, in association with other factors. -
Involvement in disease
Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
Induced at programmed cell death. -
Cellular localization
Membrane. - Information by UniProt
Images
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Human PD-1, Fc Tag, low endotoxin (HPLC-verified) captured on CM5 chip via anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) with an affinity constant of 3.6 µM as determined in a SPR assay (Biacore T200).
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The purity of Human PD-1, Fc Tag, low endotoxin (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
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Flow Cytometry assay shows that Human PD-1, Fc Tag, low endotoxin (HPLC-verified) can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 µg/mL.
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Immobilized Biotinylated Human PD-L2, Avitag,His Tag (recommended for biopanning) at 1 µg/mL (100 µL/well) on streptavidin precoated (0.2 µg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) with a linear range of 0.1-1 ng/mL.
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Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) at 2 µg/mL (100 µL/well) can bind Biotinylated Human PD-L2, Fc,Avitag with a linear range of 0.5-16 ng/mL.
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Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin with a linear range of 0.1-3 ng/mL (QC tested).
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Loaded Human PD-1, Fc Tag, low endotoxin (HPLC-verified) on Protein A Biosensor, can bind Human PD-L1, His Tag (HPLC & BLI verified) with an affinity constant of 5.3µM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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SDS-PAGE using ab221398 under reducing (R) condition. The gel was stained overnight with Coomassie Blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab221398 has not yet been referenced specifically in any publications.