Bioactive grade

Recombinant human PD1 protein (Fc Chimera Active) (ab221398)

Description

  • Product name

    Recombinant human PD1 protein (Fc Chimera Active)
    See all PD1 proteins and peptides
  • Biological activity

    Immobilized Human PD-L1, His Tag at 1 μg/mL ( 100 μL/well ) can bind ab221398 with a linear range of 0.1-3 ng/mL.

  • Purity

    > 95 % SDS-PAGE.
    >90% as determined by SEC-HPLC.
  • Endotoxin level

    < 0.100 Eu/g
  • Expression system

    HEK 293 cells
  • Accession

  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LDSPDRPWNPPTFSPALLVVTEGDNATFTCSFSNTSESFVLNWYRMSPSN QTDKLAAFPEDRSQPGQDCRFRVTQLPNGRDFHMSVVRARRNDSGTYLCG AISLAPKAQIKESLRAELRV TERRAEVPTAHPSPSPRPAGQFQ
    • Predicted molecular weight

      43 kDa including tags
    • Amino acids

      25 to 167
    • Additional sequence information

      This protein carries a human IgG1 Fc tag at the C-terminus.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab221398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

    HPLC

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: PBS, 5% Trehalose

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product in sterile deionized water to a final concentration of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD279
    • CD279 antigen
    • hPD 1
    • hPD l
    • hPD-1
    • hSLE1
    • PD 1
    • PD-1
    • PD1
    • PDCD 1
    • PDCD1
    • PDCD1_HUMAN
    • Programmed cell death 1
    • Programmed cell death 1 protein
    • Programmed cell death protein 1
    • Protein PD 1
    • Protein PD-1
    • SLEB2
    • Systemic lupus erythematosus susceptibility 2
    see all
  • Function

    Possible cell death inducer, in association with other factors.
  • Involvement in disease

    Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Developmental stage

    Induced at programmed cell death.
  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Human PD-1, Fc Tag, low endotoxin (HPLC-verified)��captured�on�CM5�chip�via�anti-human IgG Fc antibody, can bind Human PD-L1, His Tag (HPLC-verified) with an affinity constant of 3.6 µM as determined in a SPR assay (Biacore T200).
  • The purity of Human PD-1, Fc Tag, low endotoxin (HPLC-verified) was greater than 90% as determined by SEC-HPLC.
  • Flow Cytometry assay shows that Human PD-1, Fc Tag, low endotoxin (HPLC-verified)� can bind to 293 cell overexpressing human PD-L1. The concentration of PD-1 used is 1 µg/mL.
  • Immobilized Biotinylated Human PD-L2, Avitag,His Tag (recommended for biopanning) at 1 µg/mL (100 µL/well) on streptavidin precoated (0.2 µg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) with a linear range of 0.1-1 ng/mL.
  • Immobilized Human PD-1, Fc Tag, low endotoxin (HPLC-verified) at 2 µg/mL (100 µL/well) can bind Biotinylated Human PD-L2, Fc,Avitag with a linear range of 0.5-16 ng/mL.
  • Immobilized Biotinylated Human PD-L1, Avitag,His Tag (recommended for biopanning) at 1 µg/mL (100 µL/well) on streptavidin precoated (0.2 µg/well) plate, can bind Human PD-1, Fc Tag, low endotoxin (HPLC-verified) with a linear range of 0.1-3 ng/mL.
  • Loaded�Human�PD-1,�Fc�Tag,�low�endotoxin�(HPLC-verified)��on ProteinA Biosensor,�can�bind�Human PD-L1, His Tag (HPLC�& BLI verified) �with�an�affinity�constant�of 5.3�µM�as�determined�in�BLI�assay�(ForteBio�Octet�Red96e)�(Routinely�tested).
  • SDS-PAGE using ab221398 under reducing (R) condition. The gel was stained overnight with Coomassie Blue.

References

ab221398 has not yet been referenced specifically in any publications.

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