Description

  • Product name

    Recombinant Human PDE12 protein
  • Purity

    > 90 % SDS-PAGE.
    Purity determined by densitometry
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      100 kDa including tags
    • Amino acids

      1 to 609

Associated products

Specifications

Our Abpromise guarantee covers the use of ab107695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • 2 PDE
    • 2' 5' phosphodiesterase 12
    • 2' phosphodiesterase
    • 2',5'-phosphodiesterase 12
    • 2'-PDE
    • 2-PDE
    • 2PDE
    • DKFZp667B1218
    • Pde12
    • PDE12_HUMAN
    • Phosphodiesterase 12
    see all
  • Function

    Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Play a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the CCR4/nocturin family.
  • Information by UniProt

Images

  • SDS page analysis of ab107695

References

ab107695 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Review text: Dilutions of PDE12 (1 to 10ml) were incubated for 2h with 20µl of PDE12 assay substrate, an analogue of 2-5A dual labelled with Fam and Dabcyl. Active PDE12 is expected to cleave 2-5A molecules releasing the quencher from the Fluorophore, allowing it to fluoresce which is detected by the Flo-Star Optima (Ex 485nm and Em 520nm). Delta fluorescence (T120min-Tzero min) is used to calculate PDE12 % activity using cell lysate as positive control for PDE12.

Unfortunately there was no detectable fluorescence (Tzero and T120 were almost the same) observed after incubating PDE12 (ab107695) for 2h suggesting the protein under the storage conditions provided was not active.
Sample: Human Purified protein

Primary antibody (in addition to 'PDE12 protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used

Additional data
Abcam response: Accordingly to these results, this protein preparation has not conserved its functional activity. We thank the researcher to have participated in our testing offer. Unfortunately we have to conclude that this protein in ists current formulation seems not suitable for functional tests.

Dr. fouzia sadiq

Verified customer

Submitted Sep 16 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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