Recombinant Human PDE12 protein (ab107695)

Reviews (1)Q&A (2)

Description

  • Product name

    Recombinant Human PDE12 protein

  • Purity

    > 90 % SDS-PAGE.
    Purity determined by densitometry

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    Q6L8Q7

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      100 kDa including tags

    • Amino acids

      1 to 609

Associated products

Specifications

Our Abpromise guarantee covers the use of ab107695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.29% Sodium chloride

General Info

  • Alternative names

    • 2 PDE
    • 2' 5' phosphodiesterase 12
    • 2' phosphodiesterase
    • 2',5'-phosphodiesterase 12
    • 2'-PDE
    • 2-PDE
    • 2PDE
    • DKFZp667B1218
    • Pde12
    • PDE12_HUMAN
    • Phosphodiesterase 12
    see all

  • Function

    Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Play a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the CCR4/nocturin family.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human PDE12 protein (ab107695)
    SDS-PAGE - Recombinant Human PDE12 protein (ab107695)
    SDS page analysis of ab107695

References

ab107695 has not yet been referenced specifically in any publications.

Publishing research using ab107695? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a question

1-2 of 2 Q&A

Question

Hi there Please find attached data from the PDE12 assay (Abcam protein ab116283, recieved on the 20th Oct, 2011).   Increase in delta fluorescence over 2h suggests that PDE12 at lower concentration (0.2ug to 0.4ug) is active however its not significantly active, compared to the positive lysate (which is from HepG2 cell line and is not true representative).   I wonder if its possible to have an aliquot of the cell lysate from which your lab purified PDE12. I could then repeat the assay along with cell lysate from your lab and calculate PDE12 activity.   Please let me have your thoughts and comments.   Regards  

Read More
Answer

Thank you for your patience and for your feedback. I am very sorry to hear that the new protein does also not show enough activity for being used in an activity assay. In regards to use the whole cell extract, I am not very optimistic to be honest. I am not sure whether the lab is able to provide it, and even if so, it would be complicated and take time. More importantly however, I am not very hopeful that the lysate would have activity if the purified protein does not show activity.  and I do not have much hope that the transfected cells would give a better result. Indeed, in the cell extract there are many additional factors which could inhibit the activity of the protein. I am sorry for not being able to be more optimistic on this. I would actually suggest to search for a PDE12 protein produced in a different system. I have asked my colleagues to check with their collaborators whether there might be another PDE12 protein available - maybe even a PDE12 protein tested for functional activity. I will let you know as soon as I have received the answer. Meanwhile, can you please also submit the Abreviews for the two proteins? We would greatly appreciate if you could put there also a summary of the test procedure in the additional notes.  Thank you very much! Please let me know your thoughts and if you have any questions. I am looking forward to hear back from you.

Read More

Question

Hi there,   would it be possibe to find out estimated date of delivery so that I could plan the assays.   regs  

Read More
Answer

Thank you for your patience. I checked with the laboratory and our supply chain department, and we estimate to be able to deliver the product to you either Wednesday or Thursday of next week. I will be happy to send you another update next Monday. Please let me know if you have any questions. I wish you a good evening.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up