Recombinant human PDE1A protein (ab125661)

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SDS-PAGE - Recombinant human PDE1A protein (ab125661)
  • Functional Studies - Recombinant human PDE1A protein (ab125661)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Tags: His tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PDE1A protein

  • Biological activity

    The specific activity of ab125661 was determined to be 12250 - 12290 nmol/min/mg.

  • Purity

    > 90 % Densitometry.
    Purity was determined to be >90% by densitometry.

  • Expression system

    Baculovirus infected Sf9 cells

  • Accession

    P54750-4

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Predicted molecular weight

      64 kDa including tags

    • Amino acids

      2 to 545

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab125661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.00
    Preservative: 1.02% Imidazole
    Constituents: 1.05% MOPS, 0.002% PMSF, 0.004% DTT, 25% Glycerol (glycerin, glycerine), 1.75% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 3' 5' cyclic nucleotide phosphodiesterase
    • 5''-cyclic nucleotide phosphodiesterase 1A
    • 61 kDa Cam PDE
    • 61 kDa Cam-PDE
    • Calcium/calmodulin dependent 3' 5' cyclic nucleotide phosphodiesterase 1A
    • Calcium/Calmodulin stimulated cyclic nucleotide phosphodiesterase
    • Calcium/calmodulin-dependent 3''
    • Calmodulin-dependent Phosphodiesterase
    • Cam PDE 1A
    • Cam-PDE 1A
    • CAM-PDE-1A
    • HCAM 1
    • hCam-1
    • HCAM1
    • HSPDE1A
    • MGC26303
    • PDE 1A
    • PDE1A
    • PDE1A_HUMAN
    • Phosphodiesterase 1A
    • Phosphodiesterase 1A calmodulin dependent
    see all

  • Function

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a higher affinity for cGMP than for cAMP.

  • Tissue specificity

    Several tissues, including brain, kidney, testes and heart.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human PDE1A protein (ab125661)
    SDS-PAGE - Recombinant human PDE1A protein (ab125661)
    SDS-PAGE analysis of ab125661.
  • Functional Studies - Recombinant human PDE1A protein (ab125661)
    Functional Studies - Recombinant human PDE1A protein (ab125661)
    Sample Phosphodiesterase Assay showing the specific activity of ab125661 as 12250 nmol/min/mg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab125661? Please let us know so that we can cite the reference in this datasheet.

ab125661 has not yet been referenced specifically in any publications.

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