Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PDE1C/PDE1 protein
See all PDE1C/PDE1 proteins and peptides
Specific Activity: ≥ 15,000 pmol/min/µg.
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assayed using a PDE1C Assay Kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198450 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Purity>= 80 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceESPTKEIEEFESNSLKYLQPEQIEKIWLRLRGLRKYKKTSQRLRSLVKQL ERGEASVVDLKKNLEYAATVLESVYIDETRRLLDTEDELSDIQSDAVPSE VRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSNMV GLSYPPAVIEALKDVDKWSFDVFSLNEASGDHALKFIFYELLTRYDLISR FKIPISALVSFVEALEVGYSKHKNPYHNLMHAADVTQTVHYLLYKTGVAN WLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAILYNDRSVLENHH LSAAYRLLQDDEEMNILINLSKDDWREFRTLVIEMVMATDMSCHFQQIKA MKTALQQPEAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQ GDREAELGLPFSPLCDRKSTMVAQSQVGFIDFIVEPTFTVLTDMTEKIVS PLIDETSQTGGTGQRRSSLNSISSSDAKRSGVKTSGSEGSAPINNSVISV DYKSFKATWTEVVHINRERWRAKVPKEEKAKKEAEEKARLAAEEQQKEME AKSQAEEGASGKAEKKTSGETKNQVNGTRANKSDNPRGKNSKAEKSSGEQ QQNGDFKDGKNKTDKKDHSNIGNDSKKTDDSQE
Predicted molecular weight99 kDa including tags
Amino acids2 to 634
TagsGST tag N-Terminus
Additional sequence informationNM_005020.
Our Abpromise guarantee covers the use of ab198450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as PDE1C
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.02% Potassium chloride, 20% Glycerol, 0.04% Tween, 0.64% Sodium chloride, 0.05% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic nucleotide phosphodiesterase 1C
- Calcium/calmodulin dependent 3'5' cyclic nucleotide phosphodiesterase 1C
- Calcium/calmodulin-dependent 3''
FunctionCyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a high affinity for both cAMP and cGMP.
Tissue specificityExpressed in several tissues, including brain and heart.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE1 subfamily.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198450 has not yet been referenced specifically in any publications.