Recombinant human PDE2A protein (ab125613)

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Overview

Description

  • Nature

    Recombinant

  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      O00408

    • Species

      Human

    • Molecular weight

      130 kDa including tags

    • Amino acids

      2 to 941

Specifications

Our Abpromise guarantee covers the use of ab125613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab125613 was determined to be 6000 nmol/mg/min.
  • Applications

    Functional Studies

    SDS-PAGE

  • Purity

    > 70 % SDS-PAGE.
    Purity determined to be >70% by densitometry.

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 5''-cyclic phosphodiesterase
    • cGMP dependent 3'5' cyclic phosphodiesterase
    • cGMP stimulated phosphodiesterase 1
    • cGMP stimulated phosphodiesterase 4
    • cGMP-dependent 3''
    • cGMP-stimulated phosphodiesterase 2
    • CGS PDE
    • CGS-PDE
    • cGSPDE
    • Cyclic GMP stimulated phosphodiesterase
    • Cyclic GMP-stimulated phosphodiesterase
    • Human cGMP stimulated 3'5' cyclic nucleotide phosphodiesterase
    • PDE 2A
    • PDE 2A1
    • PDE 2A4
    • PDE2A
    • PDE2A_HUMAN
    • PDE2A1
    • PDE2A4
    • phosphodiesterase 2A
    • Phosphodiesterase 2A cGMP stimulated
    see all

  • Function

    Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.

  • Tissue specificity

    Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.

  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
    Contains 2 GAF domains.

  • Domain

    GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme.

  • Cellular localization

    Membrane.
  • Information by UniProt

Images

  • Functional Studies - Recombinant human PDE2A protein (ab125613)
    Functional Studies - Recombinant human PDE2A protein (ab125613)
    Sample Phosphodiesterase Assay showing the specific activity of ab125613 as 5750 nmol/mg/min.
  • SDS-PAGE - Recombinant human PDE2A protein (ab125613)
    SDS-PAGE - Recombinant human PDE2A protein (ab125613)
    SDS-PAGE analysis of ab125613.

References

ab125613 has not yet been referenced specifically in any publications.

Publishing research using ab125613? Please let us know so that we can cite the reference in this datasheet.

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