Recombinant human PDE2A protein (ab125613)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant human PDE2A protein
See all PDE2A proteins and peptides -
Biological activity
The specific activity of ab125613 was determined to be 6000 nmol/mg/min. -
Purity
> 70 % SDS-PAGE.
Purity determined to be >70% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
130 kDa including tags -
Amino acids
2 to 941
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Specifications
Our Abpromise guarantee covers the use of ab125613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic phosphodiesterase
- cGMP dependent 3'5' cyclic phosphodiesterase
- cGMP stimulated phosphodiesterase 1
see all -
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. -
Tissue specificity
Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
Contains 2 GAF domains. -
Domain
GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human PDE2A protein (ab125613)The specific activity of PDE2A (ab125613) was determined to be ~6000 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human PDE2A protein (ab125613)SDS PAGE analysis of ab125613 -
Functional Studies - Recombinant human PDE2A protein (ab125613)Sample Phosphodiesterase Assay showing the specific activity of ab125613 as 5750 nmol/mg/min. -
SDS-PAGE - Recombinant human PDE2A protein (ab125613)SDS-PAGE analysis of ab125613.
Datasheets and documents
References
ab125613 has not yet been referenced specifically in any publications.
