Recombinant human PDE2A protein (ab125613)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PDE2A protein
See all PDE2A proteins and peptides -
Biological activity
The specific activity of ab125613 was determined to be 6000 nmol/mg/min. -
Purity
> 70 % SDS-PAGE.
Purity determined to be >70% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
130 kDa including tags -
Amino acids
2 to 941
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Specifications
Our Abpromise guarantee covers the use of ab125613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic phosphodiesterase
- cGMP dependent 3'5' cyclic phosphodiesterase
- cGMP stimulated phosphodiesterase 1
see all -
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. -
Tissue specificity
Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
Contains 2 GAF domains. -
Domain
GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human PDE2A protein (ab125613)The specific activity of PDE2A (ab125613) was determined to be ~6000 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PDE2A protein (ab125613)SDS PAGE analysis of ab125613 -
Functional Studies - Recombinant human PDE2A protein (ab125613)Sample Phosphodiesterase Assay showing the specific activity of ab125613 as 5750 nmol/mg/min. -
SDS-PAGE - Recombinant human PDE2A protein (ab125613)SDS-PAGE analysis of ab125613.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125613 has not yet been referenced specifically in any publications.