Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 88% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PDE2A protein
See all PDE2A proteins and peptides
Specific Activity: ≥ 7577 pmol/min/μg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 200 μM cAMP, and ab198457 at 37ºC for 20 min. Quantified by 5-nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Purity> 88 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceGQACGHSILCRSQQYPAARPAEPRGQQVFLKPDEPPPPPQPCADSLQDAL LSLGSVIDISGLQRAVKEALSAVLPRVETVYTYLLDGESQLVCEDPPHEL PQEGKVREAIISQKRLGCNGLGFSDLPGKPLARLVAPLAPDTQVLVMPLA DKEAGAVAAVILVHCGQLSDNEEWSLQAVEKHTLVALRRVQVLQQRGPRE APRAVQNPPEGTAEDQKGGAAYTDRDRKILQLCGELYDLDASSLQLKVLQ YLQQETRASRCCLLLVSEDNLQLSCKVIGDKVLGEEVSFPLTGCLGQVVE DKKSIQLKDLTSEDVQQLQSMLGCELQAMLCVPVISRATDQVVALACAFN KLEGDLFTDEDEHVIQHCFHYTSTVLTSTLAFQKEQKLKCECQALLQVAK NLFTHLDDVSVLLQEIITEARNLSNAEICSVFLLDQNELVAKVFDGGVVD DESYEIRIPADQGIAGHVATTGQILNIPDAYAHPLFYRGVDDSTGFRTRN ILCFPIKNENQEVIGVAELVNKINGPWFSKFDEDLATAFSIYCGISIAHS LLYKKVNEAQYRSHLANEMMMYHMKVSDDEYTKLLHDGIQPVAAIDSNFA SFTYTPRSLPEDDTSMAILSMLQDMNFINNYKIDCPTLARFCLMVKKGYR DPPYHNWMHAFSVSHFCYLLYKNLELTNYLEDIEIFALFISCMCHDLDHR GTNNSFQVASKSVLAALYSSEGSVMERHHFAQAIAILNTHGCNIFDHFSR KDYQRMLDLMRDIILATDLAHHLRIFKDLQKMAEVGYDRNNKQHHRLLLC LLMTSCDLSDQTKGWKTTRKIAELIYKEFFSQGDLEKAMGNRPMEMMDRE KAYIPELQISFMEHIAMPIYKLLQDLFPKAAELYERVASNREHWTKVSHK FTIRGLPSNNSLDFLDEEYEVPDLDGTRAPINGCCSLDAE
Molecular weight information106 kDa by SDS-PAGE
Amino acids2 to 941
TagsDDDDK tag N-Terminus
Additional sequence informationGenBank Accession No. NM_002599
Our Abpromise guarantee covers the use of ab198457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
80 µg/ml DDDDK peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase
- cGMP dependent 3'5' cyclic phosphodiesterase
- cGMP stimulated phosphodiesterase 1
FunctionCyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes.
Tissue specificityExpressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
Contains 2 GAF domains.
DomainGAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab198457 has not yet been referenced specifically in any publications.