Recombinant human PDE3A protein (ab125545)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PDE3A protein
See all PDE3A proteins and peptides -
Biological activity
The specific activity of ab125545 was determined to be 500 nmol/min/mg as per activity assay protocol. -
Purity
> 85 % SDS-PAGE.
Assessed by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
84 kDa -
Amino acids
669 to 1141 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab125545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic phosphodiesterase A
- cAMP phosphodiesterase myocardial cGMP inhibited
- CGI PDE
see all -
Function
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant human PDE3A protein (ab125545)The specific activity of PDE3A (ab125545) was determined to be 550 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human PDE3A protein (ab125545)SDS PAGE analysis of ab125545 -
SDS-PAGE - Recombinant human PDE3A protein (ab125545)SDS PAGE analysis of ab125545 -
SDS-PAGE - Recombinant human PDE3A protein (ab125545)SDS-PAGE analysis of ab125545. -
Functional Studies - Recombinant human PDE3A protein (ab125545)The specific activity of ab125545 was determined to be 500nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125545 has not yet been referenced specifically in any publications.