Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 82% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PDE4B protein (Active)
See all PDE4B proteins and peptides
Biological activitySpecific Activity: ≥ 12,300 pmol/min/µg.Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.Assay Conditions: Assayed using BPS's PDE4B2 Assay Kit. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B2 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Purity>= 82 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight91 kDa including tags
TagsHis tag C-Terminus , GST tag N-Terminus
Our Abpromise guarantee covers the use of ab271679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 20% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase 4B
- cAMP-specific 3''
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B
FunctionHydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Tissue specificityExpressed in brain, heart, lung and skeletal muscle.
PathwayPurine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271679 has not yet been referenced specifically in any publications.