Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameRecombinant human PDE4D3 protein
Biological activitySpecific Activity: 3703 pmole/min/µg.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab80332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 30% Glycerol, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
RelevanceCyclic AMP-dependent phosphodiesterase type D (PDE4D) family is comprised of 5 variants (PDE4D1, D2, D3, D4 and D5). One or more PDE4D subtype-variants are ubiquitously present in all mammalian cells. In CNS all five PDE4D subtype-variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Peripheral tissues also exhibit differential expression of PDE4D variants. PDE4D1/D2 mRNA levels rise in response to an increase in cAMP. Short-term regulation of PDE4D variants involved PKA, MAP kinases and Erk2 phosphorylation that results in rapid change in their enzymatic activities. Other regulatory mechanism involved protein-protein interactions with cytoskeletal scaffolding proteins.
Cellular localizationCell Membrane and Cytoplasmic
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab80332 has not yet been referenced specifically in any publications.