Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human PDE5A/PDE5 protein
See all PDE5A/PDE5 proteins and peptides
Biological activityThe specific activity of ab125581 was determined to be 2275 nmol/min/mg.
Purity> 90 % Densitometry.
The purity was determined to be 95% by densitometry
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight63 kDa including tags
Amino acids537 to 875
TagsGST tag N-Terminus
Our Abpromise guarantee covers the use of ab125581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product was previously labelled as PDE5A
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.002% PMSF, 0.5% Magnesium chloride, 0.003% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 5''-cyclic phosphodiesterase
- CGB PDE
FunctionPlays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
Tissue specificityExpressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
PathwayPurine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
Sequence similaritiesBelongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains.
DomainComposed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
modificationsPhosphorylation is regulated by binding of cGMP to the two allosteric sites.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125581 has been referenced in 1 publication.
- Chau Y et al. Exploration of icariin analog structure space reveals key features driving potent inhibition of human phosphodiesterase-5. PLoS One 14:e0222803 (2019). PubMed: 31539416