Recombinant human PDE5A/PDE5 protein (ab125581)
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Description
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Product name
Recombinant human PDE5A/PDE5 protein
See all PDE5A/PDE5 proteins and peptides -
Biological activity
The specific activity of ab125581 was determined to be 2275 nmol/min/mg. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
63 kDa including tags -
Amino acids
537 to 875
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab125581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as PDE5A
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.5% Magnesium chloride, 0.003% DTT, 0.79% Tris HCl, 25% Glycerol, 0.29% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic phosphodiesterase
- CGB PDE
- CGB-PDE
see all -
Function
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. -
Tissue specificity
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung. -
Pathway
Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
Domain
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B. -
Post-translational
modificationsPhosphorylation is regulated by binding of cGMP to the two allosteric sites. - Information by UniProt
Images
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Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)The specific activity of PDE5A/PDE5 (ab125581) was determined to be 1940 nmol/min/mg as per activity assay protocol -
SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)SDS PAGE analysis of ab125581 -
SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)SDS PAGE analysis of ab125581 -
SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)SDS Page analysis of ab125581 -
Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)The specific activity of ab125581 was determined to be 2275 nmol/min/mg.
Datasheets and documents
References
ab125581 has not yet been referenced specifically in any publications.
