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  1. Link

    recombinant-human-pde5apde5-protein-ab125581.pdf

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Signal Transduction Second Messenger Nucleotide Messenger cGMP
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Recombinant human PDE5A/PDE5 protein (ab125581)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)
  • SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
  • SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
  • SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
  • Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 90% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies, SDS-PAGE

Description

  • Product name

    Recombinant human PDE5A/PDE5 protein
    See all PDE5A/PDE5 proteins and peptides
  • Biological activity

    The specific activity of ab125581 was determined to be 2275 nmol/min/mg.
  • Purity

    > 90 % Densitometry.
    The purity was determined to be 95% by densitometry
  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    O76074
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      63 kDa including tags
    • Amino acids

      537 to 875
    • Tags

      GST tag N-Terminus

Associated products

  • Related Products

    • Anti-PDE5A/PDE5 antibody (ab28761)
    • Anti-PDE5A/PDE5 antibody (ab64179)

Specifications

Our Abpromise guarantee covers the use of ab125581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

  • Form

    Liquid
  • Additional notes

     This product was previously labelled as PDE5A

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.002% PMSF, 0.5% Magnesium chloride, 0.003% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • 5''-cyclic phosphodiesterase
    • CGB PDE
    • CGB-PDE
    • CGBPDE
    • cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase
    • cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase
    • cGMP binding cGMP specific phosphodiesterase
    • cGMP binding/cGMP specific phosphodiesterase
    • cGMP specific 3'5' cyclic phosphodiesterase
    • cGMP specific phosphodiesterase PDE5A2
    • cGMP specific phosphodiesterase type 5A
    • cGMP-binding cGMP-specific phosphodiesterase
    • cGMP-specific 3''
    • CN5A
    • CN5N
    • PDE 5
    • PDE 5A
    • PDE5
    • Pde5a
    • PDE5A_HUMAN
    • PDE5A1
    • Phosphodiesterase 5A
    • Phosphodiesterase 5A cGMP specific
    • Phosphodiesterase isozyme 5
    see all
  • Function

    Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP.
  • Tissue specificity

    Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
  • Pathway

    Purine metabolism; 3',5'-cyclic GMP degradation; GMP from 3',5'-cyclic GMP: step 1/1.
  • Sequence similarities

    Belongs to the cyclic nucleotide phosphodiesterase family.
    Contains 2 GAF domains.
  • Domain

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
  • Post-translational
    modifications

    Phosphorylation is regulated by binding of cGMP to the two allosteric sites.
  • Target information above from: UniProt accession O76074 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)
    Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)
    The specific activity of PDE5A/PDE5 (ab125581) was determined to be 1940 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
    SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
    SDS PAGE analysis of ab125581
  • SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
    SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
    SDS PAGE analysis of ab125581
  • SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
    SDS-PAGE - Recombinant human PDE5A/PDE5 protein (ab125581)
    SDS Page analysis of ab125581
  • Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)
    Functional Studies - Recombinant human PDE5A/PDE5 protein (ab125581)
    The specific activity of ab125581 was determined to be 2275 nmol/min/mg.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab125581? Please let us know so that we can cite the reference in this datasheet.

ab125581 has been referenced in 1 publication.

  • Chau Y  et al. Exploration of icariin analog structure space reveals key features driving potent inhibition of human phosphodiesterase-5. PLoS One 14:e0222803 (2019). PubMed: 31539416

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