Recombinant human PDE7A/HCP1 protein (ab125786)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human PDE7A/HCP1 protein
See all PDE7A/HCP1 proteins and peptides -
Biological activity
The specific activity of ab125786 was determined to be 166 nmol/min/mg.
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Purity
> 85 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSSNSL NILDDDYNGQ AKCMLEKVGN WNFDIFLFDR LTNGNSLVSL TFHLFSLHGL IEYFHLDMMK LRRFLVMIQE DYHSQNPYHN AVHAADVTQA MHCYLKEPKL ANSVTPWDIL LSLIAAATHD LDHPGVNQPF LIKTNHYLAT LYKNTSVLEN HHWRSAVGLL RESGLFSHLP LESRQQMETQ IGALILATDI SRQNEYLSLF RSHLDRGDLC LEDTRHRHLV LQMALKCADI CNPCRTWELS KQWSEKVTEE FFHQGDIEKK YHLGVSPLCD RHTESIANIQ IGFMTYLVEP LFTEWARFSN TRLSQTMLGH VGLNKASWKG LQREQSSSED TDAAFELNSQ LLPQENRLS -
Predicted molecular weight
64 kDa including tags -
Amino acids
104 to 456 -
Tags
GST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab125786 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as PDE7A
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.002% PMSF, 0.4% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 5''-cyclic phosphodiesterase 7A
- HCP 1
- HCP1
see all -
Function
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction. -
Tissue specificity
PDE7A1 is found at high levels in skeletal muscle and at low levels in a variety of tissues including brain and heart. It is expressed as well in two T-cell lines. PDE7A2 is found abundantly in skeletal muscle and at low levels in heart. -
Pathway
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
Sequence similarities
Belongs to the cyclic nucleotide phosphodiesterase family. PDE7 subfamily. -
Developmental stage
Developmentally regulated. PDE7A1 and PDE7A2 are found in several fetal tissues, expression is reduced throughout development. It persists strongly only in adult skeletal muscle. -
Domain
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain. -
Cellular localization
Cytoplasm > cytosol. PDE7A1 (57 kDa) is located mostly to soluble cellular fractions and Cytoplasm. PDE7A2 (50 kDa) is located to particulate cellular fractions. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab125786 has not yet been referenced specifically in any publications.